Author(s): Castagnini M,Picchianti M, Talluri E, Biagini M, Del Vecchio M, et al.
Among the several toxins used by pathogenic bacteria to target eukaryotic host cells, proteins that exert ADP-ribosylation activity represent a large and studied family of dangerous and potentially lethal toxins. These proteins alter cell physiology catalyzing the transfer of the ADP-ribose unit from NAD to cellular proteins involved in key metabolic pathways. In the present study, we tested the capability of four of these toxins, to ADP-ribosylate α- and β- defensins. Cholera toxin (CT) from Vibrio cholerae and heat labile enterotoxin (LT) from Escherichia coli both modified the human α-defensin (HNP-1) and β- defensin-1 (HBD1), as efficiently as the mammalian mono-ADP-ribosyltransferase-1. Pseudomonas aeruginosa exoenzyme S was inactive on both HNP-1 and HBD1. Neisseria meningitidis NarE poorly recognized HNP-1 as a substrate but it was completely inactive on HBD1. On the other hand, HNP-1 strongly influenced NarE inhibiting its transferase activity while enhancing auto-ADP-ribosylation. We conclude that only some arginine-specific ADP-ribosylating toxins recognize defensins as substrates in vitro. Modifications that alter the biological activities of antimicrobial peptides may be relevant for the innate immune response. In particular, ADP-ribosylation of antimicrobial peptides may represent a novel escape mechanism adopted by pathogens to facilitate colonization of host tissues.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/22879887
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Author(s): Schuchat A, Robinson K, Wenger JD, Harrison LH, Farley M, et al.
Author(s): Iglewski BH, Kabat D
Author(s): Locht C, Keith JM
Author(s): Masignani V,Balducci E, Serruto D, Veggi D, Aricò B, et al.
Author(s): Masignani V,Balducci E, Di Marcello F, Savino S, Serruto D, et al.
Author(s): Picchianti M, Del Vecchio M, Di Marcello F, Biagini M, Veggi D, et al.
Author(s): Del Vecchio M,Pogni R, Baratto MC, Nobbs A, Rappuoli R, et al.
Author(s): Koehler C,Carlier L, Veggi D, Balducci E, Di Marcello F, et al.
Author(s): Rohde KH, Dyer DW
Author(s): Moss J, Stanley SJ
Author(s): Moss J, Manganiello VC, Vaughan M
Author(s): Barrio JR, Secrist JA 3rd, Leonard NJ
Author(s): Moss J, Stanley SJ, Burns DL, Hsia JA, Yost DA, et al.
Author(s): Wreggett KA
Author(s): Glowacki G,Braren R, Firner K, Nissen M, Kühl M, et al.
Author(s): Weng B, Thompson WC, Kim HJ, Levine RL, Moss J
Author(s): Moss J, Stanley SJ, Morin JE, Dixon JE
Author(s): Beinert H, Holm RH, Münck E
Author(s): Miller HK,Auerbuch V
Author(s): Bricker AL, Carey VJ, Wessels MR
Author(s): Perkins-Balding D, Ratliff-Griffin M, Stojiljkovic I