Author(s): Park JS, Onodera T, Nishimura S, Thompson RF, Itohara S
The anterior interpositus nucleus (AIN) is the proposed site of memory formation of eyeblink conditioning. A large part of the underlying molecular events, however, remain unknown. To elucidate the molecular mechanisms, we examined transcriptional changes in the AIN of mice trained with delay eyeblink conditioning using microarray, quantitative real-time RT-PCR, and in situ hybridization techniques. Microarray analyses suggested that transcriptionally up-regulated gene sets were largely different between early (3-d training) and late (7-d) stages. Quantitative real-time RT-PCR aided by laser microdissection indicated that the expression of representative EARLY genes (Sgk, IkBa, and Plekhf1) peaked at 1-d training in both the paired and unpaired conditioning groups, and was maintained at a higher level in the paired group than in the unpaired group after 3-d training. In situ hybridization revealed increased expression of these genes in broad cerebellar areas, including the AIN, with no hemispheric preferences. In contrast, the expression of representative LATE genes (Vamp1, Camk2d, and Prkcd) was selectively increased in the AIN of the 7-d paired group, with dominance in the ipsilateral AIN. Increased Vamp1 mRNA expression was restricted to the ipsilateral dorsolateral hump, a subregion of the AIN. These expression patterns of two distinct subsets of genes fit well with the two-stage learning theory, which proposes emotional and motor learning phases, and support the notion that AIN has a crucial role in memory formation of eyeblink conditioning.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/16569693
Author(s): Lu X, Miyachi S, Ito Y, Nambu A, Takada M
Author(s): Lu X, Miyachi S, Takada M
Author(s): McCormick DA, Thompson RF
Author(s): Gerwig M, Haerter K, Hajjar K, Dimitrova A, Maschke M, et al.
Author(s): Logan CG, Grafton ST
Author(s): Blaxton TA, Zeffiro TA, Gabrieli JD, Bookheimer SY, Carrillo MC, et al.
Author(s): Schreurs BG, McIntosh AR, Bahro M, Herscovitch P, Sunderland T, et al.
Author(s): Molchan SE, Sunderland T, McIntosh AR, Herscovitch P, Schreurs BG
Author(s): Lu X, Hikosaka O, Miyachi S
Author(s): Gruart A , Guillazo-Blanch G, Fernandez-Mas R, Jimenez-Diaz L, Delgado-Garcia JM
Author(s): Lavond DG, Hembree TL, Thompson RF
Author(s): Skelton RW
Author(s): Steinmetz JE, Lavond DG, Ivkovich D, Logan CG, Thompson RF
Author(s): Woodruff-Pak DS, Lavond DG, Logan CG, Steinmetz JE, Thompson RF
Author(s): Swain RA, Shinkman PG, Thompson JK, Grethe JS, Thompson RF
Author(s): Chapman PF, Steinmetz JE, Sears LL, Thompson RF
Author(s): Bao S, Chen L, Kim JJ, Thompson RF
Author(s): Kleim JA, Freeman JH Jr, Bruneau R, Nolan BC, Cooper NR, et al.
Author(s): Miller MJ, Chen NK, Li L, Tom B, Weiss C, et al.
Author(s): Hikosaka O, Nakahara H, Rand MK, Lu X, Nakamura K, et al.
Author(s): Lu X, Ashe J
Author(s): Ashe J, Lungu OV, Basford, AT, Lu X
Author(s): Nawrot M, Rizzo M
Author(s): Nawrot M, Rizzo M
Author(s): Schmahmann JD, Sherman JC.
Author(s): Thier P, Haarmeier T, Treue S, Barash S
Author(s): Jokisch D, Troje NF, Koch B, Schwarz M, Daum I
Author(s): Maschke M, Gomez CM, Tuite PJ, Pickett K, Konczak J
Author(s): Ravizza SM, McCormick CA, Schlerf JE, Justus T, Ivry RB, et al.
Author(s): Haarmeier T, Thier P
Author(s): Richter S, Gerwig M, Aslan B, Wilhelm H, Schoch B, et al.
Author(s): Ritvo ER, Freeman BJ, Scheibel AB, Duong T, Robinson H, et al.