Author(s): Monstein HJ, Östholm-Balkhed A, Nilsson MV, Nilsson M, Dornbusch K, et al.
Extended-spectrum β-lactamases (ESBLs) are often mediated by bla-SHV, blaTEM and blaCTX-M genes in Enterobacteriaceae and other Gram-negative bacteria. Numerous molecular typing methods, including PCR-based assays, have been developed for their identification. To reduce the number of PCR amplifications needed we have developed a multiplex PCR assay which detects and discriminates between bla-SHV, blaTEM and blaCTX-M PCR amplicons of 747, 445 and 593 bp, respectively. This multiplex PCR assay allowed the identification of bla-SHV, blaTEM and blaCTX-M genes in a series of clinical isolates of Enterobacteriaceae with previously characterised ESBL phenotype. The presence of blaSHV, blaTEM and blaCTX-M genes was confirmed by partial DNA sequence analysis. Apparently, the universal well-established CTX-M primer pair used here to reveal plasmid-encoded blaCTX-M genes would also amplify the chromosomally located K-1 enzyme gene in all Klebsiella oxytoca strains included in the study.
Author(s): Queenan AM, Bush K
Author(s): Doumith M, Ellington MJ, Livermore DM, Woodford N
Author(s): Walsh TR
Author(s): Bradford PA
Author(s): Sidjabat HE, Silveira FP, Potoski BA, Abu-Elmagd KM, Adams- Haduch JM, et al.
Author(s): Patel G, Bonomo RA
Author(s): Fallah F, Hakemi Vala M, Hashemi A, Shams S
Author(s): Nordmann P, Naas T, Poirel L
Author(s): Haji Hashemi B, Farzanehkhah M, Dolatyar A, Imani M, Farzami MR, et al.
Author(s): Center for disease control and Prevention
Author(s): Patzer JA, Dzierzanowska D
Author(s): Lee K, Yong D, Yum JH, Lim YS, Bolmstrom A, et al.
Author(s): Paterson DL, Hujer KM, Hujer AM, Yeiser B, Bonomo MD, et al.
Author(s): Boyd DA, Tyler S, Christianson S, McGeer A, Muller MP, et al.