Multiplex Real-Time PCR Detection of Klebsiella pneumoniae Carbapenemase (KPC) and New Delhi metallo-β-lactamase (NDM-1) genes, CDC

Author(s): Center for disease control and Prevention


This procedure provides instructions for Taqman-based real-time PCR detection of blaKPC and blaNDM-1 in a single reaction from gram-negative bacteria.  The universal 16S rRNA gene is used as a control for DNA extraction and amplification for each reaction.  If desired, either KPC or NDM-1 can be assayed independently by excluding the other set of primers and probe.  Although KPC and NDM appear to be the most common carbapenemases in the United States, it is important to note that there are other less common carbapenemases, as well as other mechanisms of carbapenem resistance.
Use of trade names is for identification only and does not constitute endorsement by the Centers for Disease Control and Prevention.

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