Author(s): Choi HD, Noh WC, Park JW, Lee JM, Suh JY
Purpose: Under different culture conditions, periodontal ligament (PDL) stem cells are capable of differentiating into cementoblast-like cells, adipocytes, and collagen-forming cells. Several previous studies reported that because of the stem cells in the PDL, the PDL have a regenerative capacity which, when appropriately triggered, participates in restoring connective tissues and mineralized tissues. Therefore, this study analyzed the genes involved in mineralization during differentiation of human PDL (hPDL) cells, and searched for candidate genes possibly associated with the mineralization of hPDL cells.
Methods: To analyze the gene expression pattern of hPDL cells during differentiation, the hPDL cells were cultured in two conditions, with or without osteogenic cocktails (β-glycerophosphate, ascorbic acid and dexamethasone), and a DNA microarray analysis of the cells cultured on days 7 and 14 was performed. Reverse transcription-polymerase chain reaction was performed to validate the DNA microarray data.
Results: The up-regulated genes on day 7 by hPDL cells cultured in osteogenic medium were thought to be associated with calcium/iron/metal ion binding or homeostasis (PDE1A, HFE and PCDH9) and cell viability (PCDH9), and the down-regulated genes were thought to be associated with proliferation (PHGDH and PSAT1). Also, the up-regulated genes on day 14 by hPDL cells cultured in osteogenic medium were thought to be associated with apoptosis, angiogenesis (ANGPTL4 and FOXO1A), and adipogenesis (ANGPTL4 and SEC14L2), and the down-regulated genes were thought to be associated with cell migration (SLC16A4).
Conclusions: This study suggests that when appropriately triggered, the stem cells in the hPDL differentiate into osteoblasts/cementoblasts, and the genes related to calcium binding (PDE1A and PCDH9), which were strongly expressed at the stage of matrix maturation, may be associated with differentiation of the hPDL cells into osteoblasts/cementoblasts.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/21394295
Author(s): Rimondini L, Mele S
Author(s): Silvério KG, Benatti BB, Casati MZ, Sallum EA, Nociti FH Jr
Author(s): Morsczeck C, Schmalz G, Reichert TE, Völlner F, Galler K, et al.
Author(s): McCulloch CA, Nemeth E, Lowenberg B, Melcher AH
Author(s): Sodek J
Author(s): Somerman MJ, Archer SY, Imm GR, Foster RA
Author(s): Giannopoulou C, Cimasoni G
Author(s): Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, et al.
Author(s): Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, et al.
Author(s): Shi S, Bartold PM, Miura M, Seo BM, Robey PG, et al.
Author(s): Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, et al.
Author(s): Feng F, Akiyama K, Liu Y, Yamaza T, Wang TM, et al.
Author(s): Iwata T, Yamato M, Zhang Z, Mukobata S, Washio K, et al.
Author(s): Park JC, Kim JM, Jung IH, Kim JC, Choi SH, et al.
Author(s): Choi JK, Hwang HI, Jang YJ
Author(s): Bright R, Hynes K, Gronthos S, Bartold PM
Author(s): Tan J, Xu X, Lin J, Fan L, Zheng Y, et al.
Author(s): Bojic S, Volarevic V, Ljujic B, Stojkovic M
Author(s): Hynes K, Menicanin D, Gronthos S, Bartold PM
Author(s): Liu J, Yu F, Sun Y, Jiang B, Zhang W, et al.
Author(s): Ding G, Niu J, Wei F
Author(s): Nakajima R, Ono M, Hara ES, Oida Y, Shinkawa S, et al.
Author(s): Hakki SS, Kayis SA, Hakki EE, Bozkurt SB, Duruksu G, et al.
Author(s): Lei M, Li K, Li B, Gao LN, Chen FM, et al.
Author(s): Gronthos S, Mrozik K, Shi S, Bartold PM
Author(s): Techawattanawisal W, Nakahama K, Komaki M, Abe M, Takagi Y, et al.
Author(s): Huang CY, Pelaez D, Dominguez-Bendala J, Garcia-Godoy F, Cheung HS
Author(s): Gay IC, Chen S, MacDougall M
Author(s): Li B, Jung HJ, Kim SM, Kim MJ, Jahng JW, et al.
Author(s): Kim SH, Kim KH, Seo BM, Koo KT, Kim TI, et al.
Author(s): Yamada S, Ozawa Y, Tomoeda M, Matoba R, Matsubara K, et al.
Author(s): Van Dijk LJ, Schakenraad JM, van der Voort HM, Herkströter FM, Busscher HJ
Author(s): Lang H, Schüler N, Nolden R
Author(s): Malekzadeh R, Hollinger JO, Buck D, Adams DF, McAllister BS
Author(s): Zhao M, Jin Q, Berry JE, Nociti FH Jr, Giannobile WV, et al.
Author(s): Kim JH, Park CH, Perez RA, Lee HY, Jang JH, et al.
Author(s): Ivanovski S, Vaquette C, Gronthos S, Hutmacher DW, Bartold PM
Author(s): Fujita T, Otsuka-Tanaka Y, Tahara H, Ide T, Abiko Y, et al.
Author(s): Elçin YM, Inanç B, Elçin AE
Author(s): Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, et al.
Author(s): Ivanovski S, Haase HR, Bartold PM
Author(s): Xu Q, Li B, Yuan L, Dong Z, Zhang H, et al.
