Author(s): Lang H, Schüler N, Nolden R
Regeneration processes in the periodontium occur by the interaction of different cell populations. It is known that these cells are also capable of forming new periodontal tissue after culture in vitro. The present study investigated whether replanted cultured cells from the periodontium could contribute to attachment formation. Primary cell cultures from alveolar bone and periodontal ligament were obtained from 11 minipigs. Experimentally induced furaction and interdental defects (n = 168) were treated in groups: (a) flap surgery, replantation of alveolar bone cells, and covering of the defects with Teflon membranes (ABC group); (b) flap surgery, replantation of periodontal ligament cells and membranes (PLC group); (c) flap surgery, bone gelatin (carrier material) and membranes (BG group); (d) flap surgery and membranes (NBG group); (e) flap surgery (FS group); and (f) no treatment (NT group). The defects were clinically and histologically (polyfluorochrome labeling) assessed after 10, 30, and 90 days. In the ABC group, initial calcified tissue formation at the roots was apparent after only 8 days. Marked new formation of cementum and alveolar bone and the development of a new attachment were observed after 90 days. In the BG and the NBG groups, wound healing varied depending on membrane healing and the morphology of the defects, which led to significantly poorer and variable results. Similar results were found in the PLC group, although some defects showed extensive cementum and bone formation. Defects in the FS and the NT groups healed largely by epithelialization. The study shows that replantation of cultured alveolar bone cells leads to formation of new cementum and bone, which, in turn, leads to formation of a new attachment. It is likely that the cells stabilize the tissue formation in the defect or on the root surface in the early phase of wound healing and prevent epithelial downgrowth. Results also show that regeneration in the periodontium is determined by the availability of (precursor) cells capable of forming calcified tissues.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/9465172
Author(s): Rimondini L, Mele S
Author(s): Silvério KG, Benatti BB, Casati MZ, Sallum EA, Nociti FH Jr
Author(s): Morsczeck C, Schmalz G, Reichert TE, Völlner F, Galler K, et al.
Author(s): McCulloch CA, Nemeth E, Lowenberg B, Melcher AH
Author(s): Sodek J
Author(s): Somerman MJ, Archer SY, Imm GR, Foster RA
Author(s): Giannopoulou C, Cimasoni G
Author(s): Seo BM, Miura M, Gronthos S, Bartold PM, Batouli S, et al.
Author(s): Seo BM, Miura M, Sonoyama W, Coppe C, Stanyon R, et al.
Author(s): Shi S, Bartold PM, Miura M, Seo BM, Robey PG, et al.
Author(s): Tomokiyo A, Maeda H, Fujii S, Wada N, Shima K, et al.
Author(s): Feng F, Akiyama K, Liu Y, Yamaza T, Wang TM, et al.
Author(s): Iwata T, Yamato M, Zhang Z, Mukobata S, Washio K, et al.
Author(s): Park JC, Kim JM, Jung IH, Kim JC, Choi SH, et al.
Author(s): Choi JK, Hwang HI, Jang YJ
Author(s): Bright R, Hynes K, Gronthos S, Bartold PM
Author(s): Tan J, Xu X, Lin J, Fan L, Zheng Y, et al.
Author(s): Bojic S, Volarevic V, Ljujic B, Stojkovic M
Author(s): Hynes K, Menicanin D, Gronthos S, Bartold PM
Author(s): Liu J, Yu F, Sun Y, Jiang B, Zhang W, et al.
Author(s): Ding G, Niu J, Wei F
Author(s): Nakajima R, Ono M, Hara ES, Oida Y, Shinkawa S, et al.
Author(s): Hakki SS, Kayis SA, Hakki EE, Bozkurt SB, Duruksu G, et al.
Author(s): Lei M, Li K, Li B, Gao LN, Chen FM, et al.
Author(s): Gronthos S, Mrozik K, Shi S, Bartold PM
Author(s): Techawattanawisal W, Nakahama K, Komaki M, Abe M, Takagi Y, et al.
Author(s): Huang CY, Pelaez D, Dominguez-Bendala J, Garcia-Godoy F, Cheung HS
Author(s): Gay IC, Chen S, MacDougall M
Author(s): Li B, Jung HJ, Kim SM, Kim MJ, Jahng JW, et al.
Author(s): Kim SH, Kim KH, Seo BM, Koo KT, Kim TI, et al.
Author(s): Yamada S, Ozawa Y, Tomoeda M, Matoba R, Matsubara K, et al.
Author(s): Van Dijk LJ, Schakenraad JM, van der Voort HM, Herkströter FM, Busscher HJ
Author(s): Malekzadeh R, Hollinger JO, Buck D, Adams DF, McAllister BS
Author(s): Zhao M, Jin Q, Berry JE, Nociti FH Jr, Giannobile WV, et al.
Author(s): Kim JH, Park CH, Perez RA, Lee HY, Jang JH, et al.
Author(s): Ivanovski S, Vaquette C, Gronthos S, Hutmacher DW, Bartold PM
Author(s): Choi HD, Noh WC, Park JW, Lee JM, Suh JY
Author(s): Fujita T, Otsuka-Tanaka Y, Tahara H, Ide T, Abiko Y, et al.
Author(s): Elçin YM, Inanç B, Elçin AE
Author(s): Gronthos S, Zannettino AC, Hay SJ, Shi S, Graves SE, et al.
Author(s): Ivanovski S, Haase HR, Bartold PM
Author(s): Xu Q, Li B, Yuan L, Dong Z, Zhang H, et al.