Author(s): Al-Saleh E, Al-Harmi J, Nandakumaran M, Al-Shammari M, Al-Jassar W
Objective:Folate antagonists are widely used in the treatment of various cancerous states. Paucity of data on effect of administration of one such widely used drug, methotrexate (MTX), on the status of essential trace elements and antioxidant enzymes in pregnant women or in pregnant animals prompted us to undertake this study.
Methods:MTX at a concentration of 5 mg/kg body weight was administered intraperitoneally as single dose to pregnant Sprague-Dawley rats for three consequitive days from day 17 of pregnancy. Control group of pregnant rats received single dose of saline instead of the anti-cancer drug on all the 3 days. After receiving the third dose of drug, the treated rats and control group rats were sacrificed, 1 h after intraperitoneal injection of a cocktail of essential trace elements namely, Cu, Se and Zn administered as a single bolus dose. Blood samples were collected 30 min of trace element cocktail injection, after decapitation and concentrations of trace elements in serum samples were determined by atomic absorption spectrophotometry. Concentrations of antioxidant enzymes, such as superoxide dismutase, glutathione peroxidase and total antioxidant status were determined by specific analytical kits, using spectrophotometry.
Results:In control group(n = 6), serum concentrations of Cu, Se and Zn averaged 2330.5, 614.8 and 2773.2 microg/l, while in study group (n = 6) the concentrations of trace elements averaged 2294, 596 and 2713 microg/l, respectively. Student's t-test did not show any statistical significance (p > 0.05) between various trace element concentrations in control and treated groups. Cu:Zn ratios of control and treated group of rats did not vary significantly as well. Concentrations of superoxide dismutase, glutathione peroxidase in whole blood samples in control rats averaged 165 and 43,260 U/ml, respectively, while in MTX-treated group of animals the corresponding antioxidant enzymes averaged 330.6 and 67,101 U/ml respectively. SOD and GPX values were significantly higher in drug-treated animals compared to controls (Student's t-test, p < 0.05) However, total antioxidant activity was shown to be significantly lower (Student's t-test; p < 0.05) in the drug-treated group compared to control.
Conclusions:We report for the first time that effect of MTX administration in pregnancy is not associated with significant alteration in disposition of essential trace elements. However, the effect of drug administration on antioxidant enzyme status in pregnant women cannot be excluded while using the drug in clinical states.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/19906001
Author(s): Feldmann M, Brennan FM, Maini RN
Author(s): Jaswal S, Mehta HC, Sood AK, Kaur J
Author(s): Demirbag R, Yilmaz R, Erel O, Gultekin U, Asci D, et al.
Author(s): Ozgocmen S, Ozyurt H, Sogut S, Akyol O
Author(s): Henrotin Y, Kurz B, Aigner T
Author(s): Blake DR, Hall ND, Treby DA, Halliwell B, Gutteridge JM
Author(s): Heliövaara M, Knekt P, Aho K, Aaran RK, Alfthan G, et al.
Author(s): Mazzetti I, Grigolo B, Borzì RM, Meliconi R, Facchini A
Author(s): Shah ZA, Vohora SB
Author(s): Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, et al.
Author(s): Gutteridge JM
Author(s): Ali AM, Habeeb RA, El-Azizi NO, Khattab DA, Abo-Shady RA, et al.
Author(s): Kamanli A, Naziroqlu M, Aydilek N, Hacievliyagil C
Author(s): Sarban S, Kocyigit A, Yazar M, Isikan UE
Author(s): Hassan MQ, Hadi RA, Al-Rawi ZS, Padron VA, Stohs SJ
Author(s): Afonso V, Champy R, Mitrovic D, Collin P, Lomri A
Author(s): Mirshafiey A, Mohsenzadegan M
Author(s): McCord JM
Author(s): Gambhir JK, Lali P, Jain AK
Author(s): Mishra R, Singh A, Chandra V, Negi MP, Tripathy BC, et al.
Author(s): Pallinti V, Ganesan N, Anbazhagan M, Rajasekhar G
Author(s): Thiele GM, Duryee MJ, Anderson DR, Klassen LW, Mohring SM, et al.
Author(s): Armagan A, Uzar E, Uz E, Yilmaz HR, Kutluhan S, et al.
Author(s): Vijayakumar D, Suresh K, Manoharan S
Author(s): Cimen MY, Cimen OB, Kacmaz M, Ozturk HS, Yorgancioglu R, et al.
Author(s): Gregory EM, Fridovich I
Author(s): Sung JY, Hong JH, Kang HS, Choi I, Lim SD, et al.
Author(s): Lin MT, Beal MF
Author(s): Zaghloul N, Patel H, Codipilly C, Marambaud P, Dewey S, et al.
Author(s): Igari T, Kaneda H, Horiuchi S, Ono S
Author(s): Mateen S, Moin S, Khan AQ, Zafar A, Fatima N
Author(s): Taysi S, Polat F, Gul M, Sari RA, Bakan E
Author(s): Veselinovic M, Barudzic N, Vuletic M, Zivkovic V, Tomic-Lucic A, et al.
Author(s): Benhamou PY, Moriscot C, Richard MJ, Beatrix O, Badet L, et al.
Author(s): García-González A, Gaxiola-Robles R, Zenteno-Savín T
Author(s): Feijóo M, Túnez I, Ruiz A, Tasset I, Muñoz E, et al.
Author(s): Evelo CTA, Palmen NGM, Artur Y, Janssen GME
Author(s): Bazzichi L, Ciompi ML, Betti L, Rossi A, Melchiorre D, et al.
Author(s): Kerimova AA, Atalay M, Yusifov EY, Kuprin SP, Kerimov TM
Author(s): Mulherin DM, Thurnham DI, Situnayake RD
Author(s): Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C, Raducha M, et al.
Author(s): Gum JR, Hicks JW, Sack TL, Kim YS
Author(s): Thompson PW, Houghton BJ, Clifford C, Jones DD, Whitaker KB, et al.
Author(s): Nanke Y, Kotake S, Akama H, Kamatani N
Author(s): Spooner RJ, Smith DH, Bedford D, Beck PR
Author(s): Paredes S, Girona J, Hurt-Camejo E, Vallvé JC, Olivé S, et al.
Author(s): Jalili M, Kolahi S, Aref-Hosseini SR, Mamegani ME, Hekmatdoost A