Author(s): Bazzichi L, Ciompi ML, Betti L, Rossi A, Melchiorre D, et al.
Objective:To test the activity of elastase, collagenase and glutathione reductase in the synovial fluid (SF) of patients with rheumatoid arthritis (RA) and in patients with osteoarthritis (OA); to correlate the elastase and collagenase activity with the glutathione reductase activity, which is important for the inactivation of oxygen free radicals.
Methods:24 patients affected by osteoarthrosis and 24 patients affected by rheumatoid arthritis took part in the study. We measured elastase activity towards the substrate metoxysuccinyl-alanyl-alanyl-prolyl-valyl-p-nitroanilide (MeOSuc-ala-ala-proval-p-NA) which is highly specific for elastase, and insensitive to the other serine proteases, such as cathepsin G; collagenase activity was measured using [14C]-acetylated collagen as the substrate. Glutathione reductase activity was measured following the oxidation of nicotinamide adenine dinucleotide phosphate reduced (NADPH) in the presence of oxidized glutathione (GSSG).
Results:The concentrations of elastase, collagenase and glutathione reductase were statistically higher in patients with RA than in patients with OA. Moreover, in the SF of patients with RA we found positive correlation between enzyme activity levels.
Conclusion:These results confirm a high activity of collagenase and elastase in the SF of patients with RA, which is about 30 times higher than that found in the SF of patients with OA. These data underline the synergic action of these enzymes in the pathogenesis of joint damage. RA patients also exhibit higher levels of glutathione reductase, which is important for the detoxification pathway of oxygen free radicals. However, compared with findings for collagenase and elastase, the increase in glutathione reductase is only three times higher than level found in the SF of OA patients. The limited increase in glutathione reductase activity during the inflammatory process might lead to an insufficient protective effect at the joint level in rheumatoid arthritis.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/12508766
Author(s): Feldmann M, Brennan FM, Maini RN
Author(s): Jaswal S, Mehta HC, Sood AK, Kaur J
Author(s): Demirbag R, Yilmaz R, Erel O, Gultekin U, Asci D, et al.
Author(s): Ozgocmen S, Ozyurt H, Sogut S, Akyol O
Author(s): Henrotin Y, Kurz B, Aigner T
Author(s): Blake DR, Hall ND, Treby DA, Halliwell B, Gutteridge JM
Author(s): Heliövaara M, Knekt P, Aho K, Aaran RK, Alfthan G, et al.
Author(s): Mazzetti I, Grigolo B, Borzì RM, Meliconi R, Facchini A
Author(s): Shah ZA, Vohora SB
Author(s): Arnett FC, Edworthy SM, Bloch DA, McShane DJ, Fries JF, et al.
Author(s): Gutteridge JM
Author(s): Ali AM, Habeeb RA, El-Azizi NO, Khattab DA, Abo-Shady RA, et al.
Author(s): Kamanli A, Naziroqlu M, Aydilek N, Hacievliyagil C
Author(s): Sarban S, Kocyigit A, Yazar M, Isikan UE
Author(s): Hassan MQ, Hadi RA, Al-Rawi ZS, Padron VA, Stohs SJ
Author(s): Afonso V, Champy R, Mitrovic D, Collin P, Lomri A
Author(s): Mirshafiey A, Mohsenzadegan M
Author(s): McCord JM
Author(s): Gambhir JK, Lali P, Jain AK
Author(s): Mishra R, Singh A, Chandra V, Negi MP, Tripathy BC, et al.
Author(s): Pallinti V, Ganesan N, Anbazhagan M, Rajasekhar G
Author(s): Thiele GM, Duryee MJ, Anderson DR, Klassen LW, Mohring SM, et al.
Author(s): Armagan A, Uzar E, Uz E, Yilmaz HR, Kutluhan S, et al.
Author(s): Al-Saleh E, Al-Harmi J, Nandakumaran M, Al-Shammari M, Al-Jassar W
Author(s): Vijayakumar D, Suresh K, Manoharan S
Author(s): Cimen MY, Cimen OB, Kacmaz M, Ozturk HS, Yorgancioglu R, et al.
Author(s): Gregory EM, Fridovich I
Author(s): Sung JY, Hong JH, Kang HS, Choi I, Lim SD, et al.
Author(s): Lin MT, Beal MF
Author(s): Zaghloul N, Patel H, Codipilly C, Marambaud P, Dewey S, et al.
Author(s): Igari T, Kaneda H, Horiuchi S, Ono S
Author(s): Mateen S, Moin S, Khan AQ, Zafar A, Fatima N
Author(s): Taysi S, Polat F, Gul M, Sari RA, Bakan E
Author(s): Veselinovic M, Barudzic N, Vuletic M, Zivkovic V, Tomic-Lucic A, et al.
Author(s): Benhamou PY, Moriscot C, Richard MJ, Beatrix O, Badet L, et al.
Author(s): García-González A, Gaxiola-Robles R, Zenteno-Savín T
Author(s): Feijóo M, Túnez I, Ruiz A, Tasset I, Muñoz E, et al.
Author(s): Evelo CTA, Palmen NGM, Artur Y, Janssen GME
Author(s): Kerimova AA, Atalay M, Yusifov EY, Kuprin SP, Kerimov TM
Author(s): Mulherin DM, Thurnham DI, Situnayake RD
Author(s): Weiss MJ, Henthorn PS, Lafferty MA, Slaughter C, Raducha M, et al.
Author(s): Gum JR, Hicks JW, Sack TL, Kim YS
Author(s): Thompson PW, Houghton BJ, Clifford C, Jones DD, Whitaker KB, et al.
Author(s): Nanke Y, Kotake S, Akama H, Kamatani N
Author(s): Spooner RJ, Smith DH, Bedford D, Beck PR
Author(s): Paredes S, Girona J, Hurt-Camejo E, Vallvé JC, Olivé S, et al.
Author(s): Jalili M, Kolahi S, Aref-Hosseini SR, Mamegani ME, Hekmatdoost A