Author(s): Benagli C, Rossi V, Dolina M, Tonolla M, Petrini O
Background: Matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) allows rapid and reliable identification of microorganisms, particularly clinically important pathogens.
Methodology/principal findings: We compared the identification efficiency of MALDI-TOF MS with that of Phoenix®, API® and 16S ribosomal DNA sequence analysis on 1,019 strains obtained from routine diagnostics. Further, we determined the agreement of MALDI-TOF MS identifications as compared to 16S gene sequencing for additional 545 strains belonging to species of Enterococcus, Gardnerella, Staphylococcus, and Streptococcus. For 94.7% of the isolates MALDI-TOF MS results were identical with those obtained with conventional systems. 16S sequencing confirmed MALDI-TOF MS identification in 63% of the discordant results. Agreement of identification of Gardnerella, Enterococcus, Streptococcus and Staphylococcus species between MALDI-TOF MS and traditional method was high (Crohn's kappa values: 0.9 to 0.93).
Conclusions/significance: MALDI-TOF MS represents a rapid, reliable and cost-effective identification technique for clinically relevant bacteria.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/21283545
Author(s): Claydon MA, Davey SN, Edwards-Jones V, Gordon DB
Author(s): Holland RD, Wilkes JG, Rafii F, Sutherland JB, Persons CC, et al
Author(s): Demirev PA, Ho YP, Ryzhov V, Fenselau C
Author(s): Fenselau C, Demirev PA
Author(s): Carbonnelle E, Mesquita C, Bille E, Day N, Dauphin B, et al.
Author(s): Karas M, Glückmann M, Schäfer J
Author(s): Jaskolla TW, Karas M
Author(s): Van Belkum A, Welker M, Erhard M, Chatellier S
Author(s): Jones JJ, Stump MJ, Fleming RC, Lay JO Jr, Wilkins CL
Author(s): Valentine N, Wunschel S, Wunschel D, Petersen C, Wahl K
Author(s): De Respinis S, Tonolla M, Pranghofer S, Petrini L, Petrinia O, et al
Author(s): Ciardo DE, Schär G, Altwegg M, Böttger EC, Bosshard PP
Author(s): Goldenberger D, Künzli A, Vogt P, Zbinden R, Altwegg M
Author(s): Seng P, Drancourt M, Gouriet F, La Scola B, Fournier PE, et al.
Author(s): Bizzini A, Jaton K, Romo D, Bille J, Prod'hom G, et al.
Author(s): Christner M, Rohde H, Wolters M, Sobottka I, Wegscheider K, et al.
Author(s): Ferroni A, Suarez S, Beretti JL, Dauphin B, Bille E, et al.
Author(s): Doern CD, Burnham CA
Author(s): López Roa P, Sánchez Carrillo C, Marín M, Romero F, Cercenado E, et al.
Author(s): Dunne EM, Ong EK, Moser RJ, Siba PM, Phuanukoonnon S, et al.
Author(s): Bizzini A, Durussel C, Bille J, Greub G, Prod'hom G
Author(s): Anderson NW, Buchan BW, Riebe KM, Parsons LN, Gnacinski S, et al.
Author(s): Marklein G, Josten M, Klanke U, Müller E, Horré R, et al.
Author(s): Bader O, Weig M, Taverne-Ghadwal L, Lugert R, Gross U, et al.
Author(s): De Carolis E, Posteraro B, Lass-Flörl C, Vella A, Florio AR, Torelli R, et al.
Author(s): Buskirk AD, Hettick JM, Chipinda I, Law BF, Siegel PD, et al.
Author(s): Erhard M, Hipler UC, Burmester A, Brakhage AA, Wöstemeyer J
Author(s): Nenoff P, Erhard M, Simon JC, Muylowa GK, Herrmann J, et al.
Author(s): Stevenson LG, Drake SK, Murray PR
Author(s): Kok J, Thomas LC, Olma T, Chen SC, Iredell JR
Author(s): La Scola B
Author(s): Vlek AL, Bonten MJ, Boel CH
Author(s): Wüppenhorst N, Consoir C, Lörch D, Schneider C
Author(s): Gröbner S, Kempf VA
Author(s): Bernardo K, Pakulat N, Macht M, Krut O, Seifert H, et al.
Author(s): Schlebusch S, Price GR, Hinds S, Nourse C, Schooneveldt JM, et al.
Author(s): Kuhns M, Zautner AE, Rabsch W, Zimmermann O, Weig M, et al.
Author(s): Dieckmann R, Malorny B
Author(s): Reil M, Erhard M, Kuijper EJ, Kist M, Zaiss H, et al.
Author(s): Burckhardt I, Zimmermann S
Author(s): Hrabák J, Walková R, Studentová V, Chudácková E, Bergerová T
Author(s): Hooff GP, van Kampen JJ, Meesters RJ, van Belkum A, Goessens WH, et al.
Author(s): Nagy E, Becker S, Sóki J, Urbán E, Kostrzewa M
Author(s): Wybo I, Bel A de, Soetens O, Echahidi F, Vandoorslaer K, et al.
Author(s): Bittar F, Ouchenane Z, Smati F, Raoult D, Rolain J
Author(s): Szabados F, Becker K, von Eiff C, Kaase M, Gatermann S
