Value of matrix-assisted laser desorption ionization-time of flight for routine identification of viridans group streptococci causing bloodstream infections Clin Microbiol Infect In press

Author(s): López Roa P, Sánchez Carrillo C, Marín M, Romero F, Cercenado E, et al.


Phenotypic tests do not always unequivocally identify some species of viridans group streptococci (VGS). sodA sequence analysis is the most accurate method for identification, although it requires specialized personnel and has not been applied systematically in clinical microbiology laboratory routines. Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) is emerging as a rapid alternative for bacterial identification. This study assesses the ability of MALDI-TOF and the API 20 Strep system to identify VGS isolates recovered from blood cultures using sodA sequence analysis as the reference method. All clinically significant VGS isolates recovered from blood cultures between January 2007 and January 2010 were identified by sodA sequence analysis and API 20 Strep. The strains were then tested by MALDI-TOF. Agreement between API 20 Strep/MALDI-TOF and sodA sequence analysis was determined. We examined 124 clinical isolates. Sensitivities of API 20 strep and MALDI-TOF for the species level identification of VGS isolates were, respectively, as follows: 60.5% and 73.4%. Sensitivities of API 20 strep and MALDI-TOF for the group level identification were, respectively, as follows: 70% and 94.3%. The turnaround times to identify VGS isolates by sodA sequence analysis, API 20 Strep and MALDI-TOF were 12-24, 24-48 h and 15 min, respectively. API 20 Strep cannot accurately identify all isolates of VGS. MALDI-TOF appeared to be a rapid and reliable alternative for identification of VGS strains to group level, but was not able to discriminate closely related species of certain groups.

Similar Articles

The rapid identification of intact microorganisms using mass spectrometry

Author(s): Claydon MA, Davey SN, Edwards-Jones V, Gordon DB

MALDI-TOF mass spectrometry tools for bacterial identification in clinical microbiology laboratory

Author(s): Carbonnelle E, Mesquita C, Bille E, Day N, Dauphin B, et al.

Investigation of MALDI-TOF and FT-MS techniques for analysis of Escherichia coli whole cells

Author(s): Jones JJ, Stump MJ, Fleming RC, Lay JO Jr, Wilkins CL

Identification of Dermatophytes by Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry

Author(s): De Respinis S, Tonolla M, Pranghofer S, Petrini L, Petrinia O, et al

Molecular diagnosis of bacterial endocarditis by broad-range PCR amplification and direct sequencing

Author(s): Goldenberger D, Künzli A, Vogt P, Zbinden R, Altwegg M

Multilocus sequence typing of Streptococcus pneumoniae by use of mass spectrometry

Author(s): Dunne EM, Ong EK, Moser RJ, Siba PM, Phuanukoonnon S, et al.

Identification of dermatophyte species causing onychomycosis and tinea pedis by MALDI-TOF mass spectrometry

Author(s): Erhard M, Hipler UC, Burmester A, Brakhage AA, Wöstemeyer J

MALDI-TOF mass spectrometry - a rapid method for the identification of dermatophyte species

Author(s): Nenoff P, Erhard M, Simon JC, Muylowa GK, Herrmann J, et al.

Carbapenemase activity detection by matrix-assisted laser desorption ionization-time of flight mass spectrometry

Author(s): Hrabák J, Walková R, Studentová V, Chudácková E, Bergerová T

Characterization of ß-lactamase enzyme activity in bacterial lysates using MALDI-mass spectrometry

Author(s): Hooff GP, van Kampen JJ, Meesters RJ, van Belkum A, Goessens WH, et al.

MALDI-TOF-MS for rapid detection of staphylococcal Panton-Valentine leukocidin

Author(s): Bittar F, Ouchenane Z, Smati F, Raoult D, Rolain J