Author(s): Adelli GR, BalguriSP, Majumdar S
The objective of the present study is to investigate the confounding effects, if any, of beta-cyclodextrins (βCDs) on corneal permeability coefficients obtained from in vitro transmembrane diffusion studies. Transcorneal permeability studies were carried out with 2-hydroxypropyl-beta-cyclodextrin (HPβCD) and randomly methylated-beta-cyclodextrin (RMβCD) at 5 and 2.5%w/v in isotonic phosphate-buffered solution (IPBS) (pH 7.4). Rabbit corneas received from Pel-Freez Biologicals® were used for these studies. Propranolol hydrochloride (PHCl) (1 mg/mL) was used as the paracellular permeability marker. A series of permeation studies were carried out with IPBS as the control, with CDs on the donor side only, CDs on the receiver side only, and CDs on both the donor and receiver sides. At the end of 1 or 3 h, corneas were collected and fixed using a solution containing 2%v/v glutaraldehyde + 2%w/v paraformaldehyde + IPBS and histological examinations were performed (Excalibur Pathology, Inc). The order of transcorneal permeability of PHCl was found to be CDs on the receiver side > control (no CDs) ≈ CDs on both the receiver and donor sides > CDs on the donor side. Histology studies revealed that the corneal epithelial and endothelial layers remained intact in the control sets. Damage to the cornea was observed in the order of CDs on the receiver side > CDs on the donor side > CDs on both sides > control. The use of CDs in solutions for in vitro permeation experiments with rabbit corneas needs to be carefully considered to avoid confounding effects in the data obtained.
Referred From: http://link.springer.com/10.1208/s12249-015-0315-z
Author(s): Lee TWY, RobinsonJR
Author(s): AdelliGR, Balguri SP, Punyamurthula N, Bhagav P, Majumdar S
Author(s): Cunha-Vaz J
Author(s): Balguri SP, Adelli GR, Majumdar S
Author(s): Macha S,Mitra AK
Author(s): Duvvuri S,Majumdar S, Mitra AK
Author(s): Hsu J
Author(s): Del Amo EM, Urtti A
Author(s): Kaur IP, Kanwar M
Author(s): Adelli GR, Hingorani T, PunyamurthulaN, Balguri SP, Majumdar S
Author(s): Kumari A, Sharma PK, Garg VK,Garg G
Author(s): SaettoneFM, Salminen L
Author(s): Köllmer M, Popescu C, Manda P,Zhou L, Gemeinhart RA
Author(s): Aburahma MH, Mahmoud AA
Author(s): Alan HBII, Theodorakis MC
Author(s): Yang Y, Manda P, Pavurala N, KhanMA, Krishnaiah YS
Author(s): Langer R
Author(s): Manda P, Angamuthu M, HiremathSR, Raman V, Murthy SN
Author(s): Miller RA, Brady JM, Cutright DE
Author(s): Kochinke F, Wong V
Author(s): Loria MJ, White SW, Robbins SA,Salmeto AL, Hymel KA, et al.
Author(s): Rahimy MH, PeymanGA, Chin SY, Golshani R, Aras C, et al.
Author(s): Smith TJ, Pearson PA, BlandfordDL, Brown JD, Goins KA, et al.
Author(s): Manda P, Hargett JK, Kiran Vaka SR,Repka MA, Narasimha Murthy S
Author(s): Kim H, Robinson MR, Lizak MJ,Tansey G, Lutz RJ, et al.
Author(s): Kim YM, Lim JO, Kim HK, Kim SY,Shin JP
Author(s): Gwon AE, Meadows D
Author(s): Okabe J, Kimura H, Kunou N, OkabeK, Kato A, et al.
Author(s): Manda P, Kushwaha AS, Kundu S,Shivakumar H, Jo SB, et al.
Author(s): MichelsonJB, Nozik RA
Author(s): Fialho SL, Behar-Cohen F,Silva-Cunha A
Author(s): Carcaboso AM, Chiappetta DA,Opezzo JA, Höcht C, Fandiño AC, et al.
Author(s): ChoonaraYE, Pillay V, Danckwerts MP, Carmichael TR, Meyer LC, et al.