Author(s): Balguri SP, Adelli GR, Majumdar S
The objective of the present study was to formulate indomethacin (IN)-loaded solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs) and to investigate their potential use in topical ocular delivery.Methods
IN SLNs (0.1% w/v) and NLCs (0.8% w/v) were prepared, characterized and evaluated. Their in vitro release and flux profiles across the cornea and sclera-choroid-RPE (trans-SCR) tissues and in vivo ocular tissue distribution were assessed. Furthermore, chitosan chloride (CS) (mol. wt. < 200 kDa), a cationic and water-soluble penetration enhancer, was used to modify the surface of the SLNs, and its effect was investigated through in vitro transmembrane penetration and in vivo distribution tissue studies.Results
For the IN-SLNs, IN-CS-SLNs and IN-NLCs, the particle size was 226 ± 5, 265 ± 8, and 227 ± 11 nm, respectively; the zeta potential was −22 ± 0.8, 27 ± 1.2, and −12.2 ± 2.3 mV, respectively; the polydispersity index (PDI) was 0.17, 0.30, and 0.23, respectively; and the entrapment efficiency (EE) was 81 ± 0.9, 91.5 ± 3.2 and 99.8 ± 0.2%, respectively. The surface modification of the SLNs with CS increased the ocular penetration of IN. The NLCs maintained significantly higher IN concentrations in all ocular tissues tested compared to the other formulations evaluated in vivo.Conclusion
The results suggest that lipid-based particulate systems can serve as viable vehicles for ocular delivery. The NLC formulations demonstrated increased drug loading capability, entrapment and delivery to anterior and posterior segment ocular tissues.
Author(s): Lee TWY, RobinsonJR
Author(s): AdelliGR, Balguri SP, Punyamurthula N, Bhagav P, Majumdar S
Author(s): Cunha-Vaz J
Author(s): Macha S,Mitra AK
Author(s): Adelli GR, BalguriSP, Majumdar S
Author(s): Duvvuri S,Majumdar S, Mitra AK
Author(s): Hsu J
Author(s): Del Amo EM, Urtti A
Author(s): Kaur IP, Kanwar M
Author(s): Adelli GR, Hingorani T, PunyamurthulaN, Balguri SP, Majumdar S
Author(s): Kumari A, Sharma PK, Garg VK,Garg G
Author(s): SaettoneFM, Salminen L
Author(s): Köllmer M, Popescu C, Manda P,Zhou L, Gemeinhart RA
Author(s): Aburahma MH, Mahmoud AA
Author(s): Alan HBII, Theodorakis MC
Author(s): Yang Y, Manda P, Pavurala N, KhanMA, Krishnaiah YS
Author(s): Langer R
Author(s): Manda P, Angamuthu M, HiremathSR, Raman V, Murthy SN
Author(s): Miller RA, Brady JM, Cutright DE
Author(s): Kochinke F, Wong V
Author(s): Loria MJ, White SW, Robbins SA,Salmeto AL, Hymel KA, et al.
Author(s): Rahimy MH, PeymanGA, Chin SY, Golshani R, Aras C, et al.
Author(s): Smith TJ, Pearson PA, BlandfordDL, Brown JD, Goins KA, et al.
Author(s): Manda P, Hargett JK, Kiran Vaka SR,Repka MA, Narasimha Murthy S
Author(s): Kim H, Robinson MR, Lizak MJ,Tansey G, Lutz RJ, et al.
Author(s): Kim YM, Lim JO, Kim HK, Kim SY,Shin JP
Author(s): Gwon AE, Meadows D
Author(s): Okabe J, Kimura H, Kunou N, OkabeK, Kato A, et al.
Author(s): Manda P, Kushwaha AS, Kundu S,Shivakumar H, Jo SB, et al.
Author(s): MichelsonJB, Nozik RA
Author(s): Fialho SL, Behar-Cohen F,Silva-Cunha A
Author(s): Carcaboso AM, Chiappetta DA,Opezzo JA, Höcht C, Fandiño AC, et al.
Author(s): ChoonaraYE, Pillay V, Danckwerts MP, Carmichael TR, Meyer LC, et al.