Aggravation of ischemia/reperfusion-induced gastric lesions in streptozotocin-diabetic rats

Author(s): Tashima K, Fujita A, Takeuchi K


We examined the influence of diabetes on ischemia/reperfusion-induced gastric damage in rats, in relation to the antioxidative system. Animals were injected with streptozotocin (STZ: 70 mg/kg, i.p.) and used after 5 weeks of diabetes with blood glucose levels of >350 mg/dl. Gastric mucosal blood flow (GMBF) was measured before, during and after 20 min of ischemia (1.5 ml bleeding per 100 g body weight from the carotid artery) followed by a 15-min reperfusion in the presence of acid (100 mM HCI). At the end of each experiment, gastric damage was observed macroscopically. GMBF was reduced by ischemia in all groups of rats, followed by a gradual return after reperfusion. Ischemia/reperfusion produced hemorrhagic lesions in normal rat stomachs in the presence of 100 mM HCl. These lesions were significantly aggravated when the animals were pretreated with diethyldithiocarbamate, an inhibitor of superoxide dismutase (SOD). Ischemia/reperfusion-induced damage was also markedly exacerbated in STZ-diabetic rats, but this aggravation was significantly suppressed by pretreatment with exogenous SOD or glutathione (GSH). Diabetic rat stomachs showed significantly less SOD activity as well as GSH content than normal rat stomachs. In addition, the deleterious influence of diabetes on the gastric ulcerogenic response to ischemia/reperfusion was significantly mitigated by decreasing the blood glucose levels by daily insulin treatment. These results suggest that the gastric mucosa of diabetic rats is more vulnerable to ischemia/reperfusion-induced injury, and the mechanism may be partly accounted for by impairment of the antioxidative system associated with a reduced SOD activity and GSH content.

Similar Articles

Peptic ulcer in diabetes mellitus

Author(s): Masuda H, Inoue S, Arakawa H, Koizumi K, Kubo N

Gastric mucosal damage in experimental diabetes in rats: role of endogenous glutathione

Author(s): Goldin E, Ardite E, Elizalde JI, Odriozola A, Panés J, et al.

Increased susceptibility of diabetic rat gastric mucosa to food deprivation during cold stress

Author(s): Korolkiewicz R, Tashima K, Kubomi M, Kato S, Takeuchi K

Gastric infection by Helicobacter pylori and antral gastritis in hyperglycemic obese and in diabetic subjects

Author(s): Perdichizzi G, Bottari M, Pallio S, Fera MT, Carbone M, et al.

4-Hydroxyisoleucine Experimental evidence of its insulinotropic and antidiabetic properties

Author(s): Broca C, Gross R, Petti P, Sauvaire Y, Manteghetti M, et al.

Antimutagenic and anticarcinogenic activity of Emblica officinalis

Author(s): Jose JK, Kuttan G, George J, Kuttan R

Protein measurement with the folin phenol reagent

Author(s): Lowry CH, Roosebrough NJ, Farr AL, Randall RJ

Combinatorial biosynthesis of novel antibiotics related to daptomycin

Author(s): Nguyen KT, Ritz D, Gu JQ, Alexander D, Chu M, et al.

Involvement of endogenous cholecystokinin and somatostatin in gastroprotection induced by intraduodenal fat

Author(s):  Brzozowski T, Konturek PC, Konturek SJ, Kwiecién S, Pajdo R, et al.

A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat

Author(s): Reed MJ, Meszaros K, Entes LJ, Claypool MD, Pinkett JG, et al.

Alpha-glucosidase inhibitory activity of Syzygium cumini (Linn

Author(s): Shinde J, Taldone T, Barletta M, Kunaparaju N, Hu B, et al.

Influence of dietary quercetin on glutathione redox status in mice

Author(s): Meyers KJ, Rudolf JL, Mitchell AE

Effect of the flavonoid quercetin on inflammation and lipid peroxidation induced by Helicobacter pylori in gastric mucosa of guinea pig

Author(s): González-Segovia R, Quintanar JL, Salinas E, Ceballos-Salazar R, Aviles-Jiménez F, et al.