Author(s): Nie R, Zhong X, Xia Z
To investigate the protective activity of tanshinone in a rat model of myocardial ischemia-reperfusion and determine its effect on the expression of Bcl-2 and Bax in cardiomyocytes.
MethodsWe established a rat model of myocardial ischemia-reperfusion. Rats were randomly divided into blank (no surgery); saline; and low-dose (2 mg/ml), medium-dose (15 mg/ml), and high-dose (50 mg/ml) tanshinone groups. We measured heart rate and troponin (cTnI) levels, performed TUNEL to detect cardiomyocyte apoptosis, and detected LDH and CK-MB activities in serum by ELISA. We performed RT-qPCR and western blot to detect the expression of Bcl-2 and Bax mRNA and protein in cardiomyocytes.
ResultsRats treated with tanshinone experienced more stable heart rate after ischemia-reperfusion compared with those in the saline control group. cTnI decreased after ischemia-reperfusion in mice injected with tanshinone, while cTnI in saline-treated mice increased significantly compared with that in the blank control group. TUNEL staining showed that there were greater apoptotic cardiomyocytes in the saline group, but the tanshinone groups showed fewer apoptotic cardiomyocytes. LDH and CK-MB activities were significantly increased after reperfusion in the saline group (p<0.01) and also in the low- and medium-dose tanshinone groups (p<0.05). However, no significant differences were found in the high-dose tanshinone group (p>0.05). The expression levels of Bcl-2 mRNA and protein in cardiomyocytes of rats were higher in the three tanshinone groups in a dose-sensitive manner than those in the blank and saline groups (p<0.05). By contrast, the expression levels of Bax mRNA and protein were reduced in the three tanshinone groups in a dose-sensitive manner compared to those in the blank and saline groups (p<0.05).
ConclusionTanshinone shows a protective effect in a dose-dependent manner in a rat model of myocardial ischemia-reperfusion, suggesting its potential therapeutic use.
Author(s): Dhingra R, Vasan RS
Author(s): Fox KA, Bergmann SR, Sobel BE
Author(s): Luca MC, Liuni A, Muxel S, Münzel T, Forconi S, et al.
Author(s): Xia Z, Xia R, Lan HT, Luo T, Tang QZ, et al.
Author(s): Fan Q, Yang XC, Liu Y, Wang LF, Liu SH, et al.
Author(s): Huang Z, Zhong X, Irwin MG, Ji S, Wong GT, et al.
Author(s): Xia Z, Wang F, Luo T
Author(s): Gong JS, Yao YT, Fang NX, Huang J, Li LH
Author(s): Liu C, Dai R, Yu R, Xu J
Author(s): Wang T, Qiao S, Lei S, Liu Y, Ng KF, et al.
Author(s): Balakumar P, Sharma NK
Author(s): Tai W, Shi E, Yan L, Jiang X, Ma H, et al.
Author(s): Lopaschuk GD, Ussher JR, Folmes CD, Jaswal JS, Stanley WC
Author(s): Buja LM
Author(s): Kim DH, Kim YJ, Chang SA, Lee HW, Kim HN, et al.
Author(s): Greer EL, Brunet A
Author(s): Vogt PK, Jiang H, Aoki M
Author(s): Maiese K, Chong ZZ, Shang YC, Hou J
Author(s): Sengupta A, Molkentin JD, Paik JH, DePinho RA, Yutzey KE
Author(s): Zhao X, Gan L, Pan H, Kan D, Majeski M, et al.
Author(s): Van Der Heide LP, Hoekman MF, Smidt MP
Author(s): Oellerich MF, Potente M
Author(s): Ferdous A, Battiprolu PK, Ni YG, Rothermel BA, Hill JA
Author(s): Ronnebaum SM, Patterson C
Author(s): Battiprolu PK, Hojayev B, Jiang N, Wang ZV, Luo X, et al.
Author(s): Puthanveetil P, Zhang D, Wang Y, Wang F, Wan A, et al.
Author(s): Puthanveetil P, Wang Y, Wang F, Kim MS, Abrahani A, et al.
Author(s): Puthanveetil P, Wang Y, Zhang D, Wang F, Kim MS, et al.
Author(s): Miki T, Itoh T, Sunaga D, Miura T
Author(s): Akiyama N, Okumura K, Watanabe Y, Hashimoto H, Ito T, et al.
Author(s): Nakada T, Kwee IL
Author(s): Depre C, Vanoverschelde JL, Taegtmeyer H
Author(s): Wijns W, Vatner SF, Camici PG
Author(s): Leung AA, Eurich DT, Lamb DA, Majumdar SR, Johnson JA, et al.
Author(s): Huffmyer J, Raphael J
Author(s): Tremblay ML, Giguère V
Author(s): Steinberg SF
Author(s): Furuyama T, Kitayama K, Yamashita H, Mori N
Author(s): Ding M, Rzucidlo EM, Davey JC, Xie Y, Liu R, et al.
Author(s): Gross DN, van den Heuvel AP, Birnbaum MJ
Author(s): Marfella R, Di Filippo C, Esposito K, Nappo F, Piegari E, et al.
Author(s): Tzivion G, Dobson M, Ramakrishnan G
Author(s): Kim MS, Wang F, Puthanveetil P, Kewalramani G, Innis S, et al.
Author(s): Parisi Q, Biondi-Zoccai GG, Abbate A, Santini D, Vasaturo F, et al.
Author(s): Andrews NP, Prasad A, Quyyumi AA
Author(s): Brunet J, Boily MJ, Cordeau S, Des Rosiers C
Author(s): Hsu CP, Zhai P, Yamamoto T, Maejima Y, Matsushima S, et al.
Author(s): Brunet A, Sweeney LB, Sturgill JF, Chua KF, Greer PL, et al.
Author(s): Pitocco D, Zaccardi F, Di Stasio E, Romitelli F, Santini SA, et al.
Author(s): Mehra A, Shotan A, Ostrzega E, Hsueh W, Vasquez-Johnson J, et al.
Author(s): Bauters C, Lamblin N, Mc Fadden EP, Van Belle E, Millaire A, et al.
Author(s): Zhao ZQ, Corvera JS, Halkos ME, Kerendi F, Wang NP, et al.
Author(s): Kok BP, Brindley DN
Author(s): Philip-Couderc P, Tavares NI, Roatti A, Lerch R, Montessuit C, et al.
Author(s): Xuan YT, Guo Y, Han H, Zhu Y, Bolli R
Author(s): Tsang A, Hausenloy DJ, Mocanu MM, Carr RD, Yellon DM
Author(s): Zhan L, Wang T, Li W, Xu ZC, Sun W, et al.
Author(s): Somers SJ, Frias M, Lacerda L, Opie LH, Lecour S
