Gastric mucosal damage in experimental diabetes in rats: role of endogenous glutathione

Author(s): Goldin E, Ardite E, Elizalde JI, Odriozola A, Panés J, et al.


Background & aims: Spontaneous gastric damage occurs in diabetic rats, but the mechanism is unknown. The aim of this study was to assess the role of glutathione metabolism and gastric mucosal blood flow (GMBF) in the development of such spontaneous gastric damage.

Methods: Mucosal damage, GMBF, glutathione metabolism, and lipid peroxidation were measured in the stomach of diabetic and control rats.

Results: Spontaneous gastric damage occurred in fasted diabetic rats 4 weeks after streptozotocin administration or pancreatectomy. This was accompanied by a 50% decrement in mucosal content of glutathione; 48 hours after streptozotocin, the decrement of glutathione was only of 25% and no gastric damage was observed. Fed diabetic rats (4 weeks after streptozotocin) had normal glutathione levels and no damage; however, a 30% glutathione depletion achieved by buthionine-sulfoximine administration promoted significant damage. Gastric glutathione synthetic rate, levels of adenosine triphosphate, oxidized glutathione, and malonyldialdehyde were similar in all groups, whereas cysteine concentration was reduced in fasted diabetic animals. Exogenous cysteine attenuated the gastric damage. GMBF was not influenced by diabetes.

Conclusions: Spontaneous gastric damage in fasted diabetic rats seems to be related to glutathione depletion as a result of limited availability of cysteine and not to increased glutathione oxidation. GMBF changes are not involved.

Similar Articles

Peptic ulcer in diabetes mellitus

Author(s): Masuda H, Inoue S, Arakawa H, Koizumi K, Kubo N

Increased susceptibility of diabetic rat gastric mucosa to food deprivation during cold stress

Author(s): Korolkiewicz R, Tashima K, Kubomi M, Kato S, Takeuchi K

Gastric infection by Helicobacter pylori and antral gastritis in hyperglycemic obese and in diabetic subjects

Author(s): Perdichizzi G, Bottari M, Pallio S, Fera MT, Carbone M, et al.

4-Hydroxyisoleucine Experimental evidence of its insulinotropic and antidiabetic properties

Author(s): Broca C, Gross R, Petti P, Sauvaire Y, Manteghetti M, et al.

Antimutagenic and anticarcinogenic activity of Emblica officinalis

Author(s): Jose JK, Kuttan G, George J, Kuttan R

Protein measurement with the folin phenol reagent

Author(s): Lowry CH, Roosebrough NJ, Farr AL, Randall RJ

Combinatorial biosynthesis of novel antibiotics related to daptomycin

Author(s): Nguyen KT, Ritz D, Gu JQ, Alexander D, Chu M, et al.

Involvement of endogenous cholecystokinin and somatostatin in gastroprotection induced by intraduodenal fat

Author(s):  Brzozowski T, Konturek PC, Konturek SJ, Kwiecién S, Pajdo R, et al.

A new rat model of type 2 diabetes: the fat-fed, streptozotocin-treated rat

Author(s): Reed MJ, Meszaros K, Entes LJ, Claypool MD, Pinkett JG, et al.

Alpha-glucosidase inhibitory activity of Syzygium cumini (Linn

Author(s): Shinde J, Taldone T, Barletta M, Kunaparaju N, Hu B, et al.

Influence of dietary quercetin on glutathione redox status in mice

Author(s): Meyers KJ, Rudolf JL, Mitchell AE

Effect of the flavonoid quercetin on inflammation and lipid peroxidation induced by Helicobacter pylori in gastric mucosa of guinea pig

Author(s): González-Segovia R, Quintanar JL, Salinas E, Ceballos-Salazar R, Aviles-Jiménez F, et al.