Author(s): Su CF, Chang YY, Pai HH, Liu IM, Lo CY, et al.
Background: Aerobic exercise including treadmill running has long been used to successfully treat and/or prevent insulin resistance and type-2 diabetes. Increase of plasma beta-endorphin is observed with exercise. The present study was designed to clarify the role of endogenous beta-endorphin in exercise-induced improvement in insulin resistance.
Methods: We used a moderate exercise program consisting of treadmill running at 20 m/min and 0% grade for 1 h/day, 7 days/week, for 8 weeks. Plasma glucose concentration was assessed by the glucose oxidase method. The enzyme-linked immunosorbent assay was performed to quantify the plasma level of beta-endorphin-like immunoreactivity (BER). The glucose disposal rate (GDR) was measured by the hyperinsulinemic euglycemic clamp technique. Changes of the insulin signaling in isolated soleus muscle were then detected by immunoprecipitation and immunoblotting.
Results: An increase of plasma BER in parallel with the reduction of plasma glucose was obtained in exercise-trained obese Zucker rats. Different from a marked reduction in sedentary obese rats, the value of insulin-stimulated GDR obtained from the exercised obese rats was reversed to near that of the sedentary lean group, eight weeks after the last period of exercise. This effect of exercise was inhibited by naloxone or naloxonazine at doses sufficient to block opioid micro-receptors. Signaling-related defects in the soleus muscle of sedentary obese Zucker rats, which impaired glucose transporter subtype 4 (GLUT 4), included decreased phosphorylation of insulin receptor substrate (IRS)-1, as well as an attenuated p85 regulatory subunit of phosphatidylinositol 3-kinase (PI3 kinase) and Akt serine phosphorylation. In contrast, exercise training failed to modify the levels of insulin receptor (IR), IRS-1, and IR tyrosine autophosphorylation in obese Zucker rats.
Conclusion: Enhanced insulin sensitivity via exercise training might be mediated by endogenous beta-endorphin through an increase of postreceptor insulin signaling related to the IRS-1-associated PI3-kinase step that leads to the enhancement of GLUT 4 translocation and improved glucose disposal in obese Zucker rats.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/15386812
Author(s): Jedrzejuk D, Medras M, Milewicz A, Demissie M
Author(s): Jessen N, Pold R, Buhl ES, Jensen LS, Schmitz O, et al.
Author(s): Zierath JR
Author(s): Cartee GD, Young DA, Sleeper MD, Zierath J, Wallberg-Henriksson H, et al.
Author(s): Kim Y, Inoue T, Nakajima R, Nakae K, Tamura T, et al.
Author(s): Labrie F, Luu-The V, Bélanger A, Lin SX, Simard J, et al.
Author(s): Aizawa K, Iemitsu M, Maeda S, Jesmin S, Otsuki T, et al.
Author(s): Sato K, Iemitsu M, Aizawa K, Ajisaka R
Author(s): Yamaguchi Y, Tanaka S, Yamakawa T, Kimura M, Ukawa K, et al.
Author(s): Aizawa K, Iemitsu M, Maeda S, Mesaki N, Ushida T, et al.
Author(s): Sato K, Iemitsu M, Aizawa K, Mesaki N, Fujita S
Author(s): Sato K, Iemitsu M, Aizawa K, Ajisaka R
Author(s): Aizawa K, Iemitsu M, Otsuki T, Maeda S, Miyauchi T, et al.
Author(s): Aizawa K, Iemitsu M, Maeda S, Otsuki T, Sato K, et al.
Author(s): Etgen GJ, Oldham BA
Author(s): Tokuyama Y, Sturis J, DePaoli AM, Takeda J, Stoffel M, et al.
Author(s): Park JH, Iemitsu M, Maeda S, Kitajima A, Nosaka T, et al.
Author(s): Searls YM, Loganathan R, Smirnova IV, Stehno-Bittel L
Author(s): Iemitsu M, Miyauchi T, Maeda S, Tanabe T, Takanashi M, et al.
Author(s): Berdanier CD, Parente JA Jr, McIntosh MK
Author(s): Muller M, Grobbee DE, den Tonkelaar I, Lamberts SW, van der Schouw YT
Author(s): Henriksen EJ, Teachey MK, Lindborg KA, Diehl CJ, Beneze AN
Author(s): Sakamoto S, Minami K, Niwa Y, Ohnaka M, Nakaya Y, et al.
Author(s): Bhasin S, Travison TG, Storer TW, Lakshman K, Kaushik M, et al.
