NRF2, a member of the NFE2 family of transcription factors, is not essential for murine erythropoiesis, growth, and development

Author(s): Chan K, Lu R, Chang JC, Kan YW


The locus control region of the beta-globin gene is composed of four erythroid-specific hypersensitive sites. Hypersensitive site 2 has been shown to be a powerful enhancer and contains a tandem repeat sequence for the transcription factors AP1 and NFE2 (activating protein 1 and nuclear factor erythroid 2, respectively). The human NRF2 (NFE2 related factor 2) has been isolated by bacterial expression screening using this core sequence as a probe. p45-NFE2, NRF1, and NRF2 belong to the CNC ("cap 'n' collar") subfamily of the basic region-leucine zipper transcription factors, which exhibits strong homology at specific regions such as the "CNC" and the DNA binding and leucine zipper domains. Although the erythroid-specific p45-NFE2 has been implicated in globin gene regulation, p45-NFE2 null mice succumb to bleedings due to lack of platelets and those that survive exhibit only a mild anemia. To determine the function of NRF2, which we found to be widely expressed in vivo, we have characterized the genomic structure of the mouse NRF2 gene, disrupted the Nrf2 gene by homologous recombination in mouse embryonic stem cells (ES cells), and generated NRF2-/- mice. Homozygous mutant mice developed normally, were not anemic, reached adulthood, and reproduced. Our studies indicate that NRF2 is dispensable for mouse development.

Similar Articles

Global and societal implications of the diabetes epidemic

Author(s): Zimmet P, Alberti KG, Shaw J

Diabetic cardiomyopathy

Author(s): Murarka S, Movahed MR

Diabetic cardiomyopathy--a distinct disease? Best Pract Res Clin Endocrinol Metab 23: 347-360

Author(s): Khavandi K, Khavandi A, Asghar O, Greenstein A, Withers S, et al.

Oxidative stress and diabetic complications

Author(s): Giacco F, Brownlee M

Vacuolar leukoencephalopathy with widespread astrogliosis in mice lacking transcription factor Nrf2

Author(s): Hubbs AF, Benkovic SA, Miller DB, O'Callaghan JP, Battelli L, et al.

The transcription factor NRF2 protects against pulmonary fibrosis

Author(s): Cho HY, Reddy SP, Yamamoto M, Kleeberger SR