Author(s): Osterwalder T, Yoon KS, White BH, Keshishian H
In Drosophila, the most widely used system for generating spatially restricted transgene expression is based on the yeast GAL4 protein and its target upstream activating sequence (UAS). To permit temporal as well as spatial control over UAS-transgene expression, we have explored the use of a conditional RU486-dependent GAL4 protein (GeneSwitch) in Drosophila. By using cloned promoter fragments of the embryonic lethal abnormal vision gene or the myosin heavy chain gene, we have expressed GeneSwitch specifically in neurons or muscles and show that its transcriptional activity within the target tissues depends on the presence of the activator RU486 (mifepristone). We used available UAS-reporter lines to demonstrate RU486-dependent tissue-specific transgene expression in larvae. Reporter protein expression could be detected 5 h after systemic application of RU486 by either feeding or "larval bathing." Transgene expression levels were dose-dependent on RU486 concentration in larval food, with low background expression in the absence of RU486. By using genetically altered ion channels as reporters, we were able to change the physiological properties of larval bodywall muscles in an RU486-dependent fashion. We demonstrate here the applicability of GeneSwitch for conditional tissue-specific expression in Drosophila, and we provide tools to control pre- and postsynaptic expression of transgenes at the larval neuromuscular junction during postembryonic life.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/11675495
Author(s): Chakraborty R, Vepuri V, Mhatre SD, Paddock BE, Miller S, et al.
Author(s): Katewa SD, Demontis F, Kolipinski M, Hubbard A, Gill MS, et al.
Author(s): Kissling C, Retz W, Wiemann S, Coogan AN, Clement RM, et al.
Author(s): Baird AL, Coogan AN, Siddiqui A, Donev RM, Thome J
Author(s): Golimstok A, Rojas JI, Romano M, Zurru MC, Doctorovich D, et al.
Author(s): Edison P, Rowe CC, Rinne JO, Ng S, Ahmed I, et al.
Author(s): Neef D1, Walling AD
Author(s): Van Gool D, De Strooper B, Van Leuven F, Dom R
Author(s): Harper DG, Stopa EG, McKee AC, Satlin A, Fish D, et al.
Author(s): vanSwinderen B, Brembs B
Author(s): Krinsky-McHale SJ, Devenny DA, Kittler P, Silverman W
Author(s): Landis SC, Amara SG, Asadullah K, Austin CP, Blumenstein R, et al.
Author(s): Zid BM, Rogers AN, Katewa SD, Vargas MA, Kolipinski MC, et al.