Author(s): Hachiya NS, Watanabe K, Yamada M, Sakasegawa Y, Kaneko K
In order to investigate the microtubule-associated intracellular trafficking of the NH2-terminal cellular prion protein (PrPC) fragment [Biochem. Biophys. Res. Commun. 313 (2004) 818], we performed a real-time imaging of fluorescent PrPC (GFP-PrPC) in living cells. Such GFP-PrPC exhibited an anterograde movement towards the direction of plasma membranes at a speed of 140-180 nm/s, and a retrograde movement inwardly at a speed of 1.0-1.2 microm/s. The anterograde and retrograde movements of GFP-PrPC were blocked by a kinesin family inhibitor (AMP-PNP) and a dynein family inhibitor (vanadate), respectively. Furthermore, anti-kinesin antibody (alpha-kinesin) blocked its anterograde motility, whereas anti-dynein antibody (alpha-dynein) blocked its retrograde motility. These data suggested the kinesin family-driven anterograde and the dynein-driven retrograde movements of GFP-PrPC. Mapping of the interacting domains of PrPC identified amino acid residues indispensable for interactions with kinesin family: NH2-terminal mouse (Mo) residues 53-91 and dynein: NH2-terminal Mo residues 23-33, respectively. Our findings argue that the discrete N-terminal amino acid residues are indispensable for the anterograde and retrograde intracellular movements of PrPC.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/14985083
Author(s): Rogers M, Serban D, Gyuris T, Scott M, Torchia T, et al.
Author(s): Pan KM, Stahl N, Prusiner SB
Author(s): Taraboulos A, Scott M, Semenov A, Avrahami D, Laszlo L, et al.
Author(s): Shyng SL, Moulder KL, Lesko A, Harris DA
Author(s): Nunziante M, Gilch S, Schätzl HM
Author(s): Lee KS, Magalhães AC, Zanata SM, Brentani RR, Martins VR, et al.
Author(s): Magalhães AC, Silva JA, Lee KS, Martins VR, Prado VF, et al.
Author(s): Negro A, Ballarin C, Bertoli A, Massimino ML, Sorgato MC
Author(s): Lorenz H, Windl O, Kretzschmar HA
Author(s): Ivanova L, Barmada S, Kummer T, Harris DA
Author(s): Butler DA, Scott MR, Bockman JM, Borchelt DR, Taraboulos A, et al.
Author(s): Pollard TD
Author(s): Howard J, Hyman AA
Author(s): Brown DR
Author(s): Collinge J, Whittington MA, Sidle KC, Smith CJ, Palmer MS, et al.
Author(s): Brown DR, Qin K, Herms JW, Madlung A, Manson J, et al.
Author(s): Santuccione A, Sytnyk V, Leshchyns'ka I, Schachner M
Author(s): Hirokawa N, Takemura R
Author(s): Hirokawa N
Author(s): Peretti D, Peris L, Rosso S, Quiroga S, Cáceres A
Author(s): Setou M, Seog DH, Tanaka Y, Kanai Y, Takei Y, et al.
Author(s): Nakata T, Hirokawa N
Author(s): Mallik R, Gross SP
Author(s): Svoboda K, Block SM
Author(s): Howard J, Hudspeth AJ, Vale RD
Author(s): Adachi N, Kohara K, Tsumoto T