Author(s): Hess DC, Hill WD, Martin-Studdard A, Carroll J, Brailer J, et al.
Background and purpose:After an ischemic event, bone marrow-derived cells may be involved in reparative processes. There is increasing evidence that bone marrow-derived stem cells may be a source of endothelial cells and organ-specific cells. Our objectives were to determine whether bone marrow-derived cells were a source of endothelial cells and neurons after cerebral ischemia.
Methods:We transplanted bone marrow from male C57 BL/6-TgN (ACTbEGFP)1Osb mice, which express green fluorescent protein (GFP), into female C57 BL/6J mice. The recipient mice then underwent suture occlusion of the middle cerebral artery (MCA), and bone marrow- derived cells were tracked by GFP epifluorescence and Y chromosome probe.
Results:Within 3 days and at 7 and 14 days after MCA occlusion, bone marrow-derived cells incorporated into the vasculature in the ischemic zone and expressed an endothelial cell phenotype. Few bone marrow-derived cells incorporated into the vasculature 24 hours after MCA occlusion. Some bone marrow-derived cells also expressed the neuronal marker NeuN at 7 and 14 days after ischemia.
Conclusions:Postnatal vasculogenesis occurs in the brain in the setting of a cerebral infarction. Bone marrow-derived cells are a source of endothelial cells and NeuN-expressing cells after cerebral infarction. This plasticity may be exploited in the future to enhance recovery after stroke.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/11988616
Author(s): Hung CW, Liou YJ, Lu SW, Tseng LM, Kao CL, et al.
Author(s): Leker RR, McKay RD
Author(s): Nakaguchi K, Masuda H, Kaneko N, Sawamoto K
Author(s): Kaneko Y, Tajiri N, Shinozuka K, Glover LE, Weinbren NL, et al.
Author(s): Uemura M, Kasahara Y, Nagatsuka K, Taguchi A
Author(s): Gage FH
Author(s): Doetsch F, Caillé I, Lim DA, García-Verdugo JM, Alvarez-Buylla A
Author(s): Iwai M, Sato K, Omori N, Nagano I, Manabe Y, et al.
Author(s): Iwai M, Sato K, Kamada H, Omori N, Nagano I, et al.
Author(s): Arvidsson A, Collin T, Kirik D, Kokaia Z, Lindvall O
Author(s): Teramoto T, Qiu J, Plumier JC, Moskowitz MA
Author(s): Gensert JM, Goldman JE
Author(s): Menn B, Garcia-Verdugo JM, Yaschine C, Gonzalez-Perez O, Rowitch D, et al.
Author(s): Ninomiya M, Yamashita T, Araki N, Okano H, Sawamoto K
Author(s): Türeyen K, Vemuganti R, Bowen KK, Sailor KA, Dempsey RJ
Author(s): Emsley JG, Hagg T
Author(s): Yan YP, Sailor KA, Vemuganti R, Dempsey RJ
Author(s): Zhu W, Cheng S, Xu G, Ma M, Zhou Z, et al.
Author(s): Kako E, Kaneko N, Aoyama M, Hida H, Takebayashi H, et al.
Author(s): Zhang ZG, Zhang L, Jiang Q, Zhang R, Davies K, et al.
Author(s): Paczkowska E, Kucia M, Koziarska D, Halasa M, Safranow K, et al.
Author(s): Beltrami AP, Barlucchi L, Torella D, Baker M, Limana F, et al.
Author(s): Asahara T, Takahashi T, Masuda H, Kalka C, Chen D, et al.
Author(s): Kalka C, Masuda H, Takahashi T, Gordon R, Tepper O, et al.
Author(s): Sehara Y, Hayashi T, Deguchi K, Zhang H, Tsuchiya A, et al.
