Author(s): Asl SZ, Khaksari M, Khachki AS, Shahrokhi N, Nourizade S
Object:Although there is evidence that estradiol has neuroprotective effects after traumatic brain injury (TBI) in female rats, it is unclear which estrogen receptor (ER) subtype, ERα or ERβ, mediates this effect. The authors therefore examined the roles of the different ERs in this effect. Here the authors used the ERα selective agonist propyl pyrazole triol (PPT) and the ERβ selective agonist diarylpropionitrile (DPN) alone and in combination in female rats to investigate this question.
Methods:Before the ovariectomized animals were injured using the Marmarou TBI technique, they were randomly divided into the following 9 groups: control, sham, TBI, vehicle, E1 (physiological dose of 17-β estradiol), E2 (pharmacological dose of 17-β estradiol), PPT, DPN, and PPT+DPN. Levels of blood-brain barrier (BBB) disruption (5 hours) and water content (24 hours) were evaluated after TBI. In groups receiving drugs or vehicle, treatment was administered as a single dose intraperitoneally 30 minutes after induction of TBI.
Results:Results showed that brain edema or brain water content after TBI was lower (p < 0.001) in the E2, PPT, DPN, and PPT+DPN groups than it was in the vehicle group. After trauma, the Evans blue dye content or BBB permeability was significantly higher in the TBI and vehicle groups (p < 0.001) than in the E2, PPT, DPN, and PPT+DPN groups. The inhibitory effects of PPT+DPN on brain water content, neurological scores, and Evans blue dye content were the highest for all groups. Although both PPT and DPN increased neurological scores after TBI, PPT appears to be more effective in increasing neurological scores.
Conclusions:Neuroprotective effects of estradiol on brain edema, BBB permeability, and neurological scores are mediated through both ERα and ERβ. This may suggest a therapeutic potential in the brain trauma for ER-specific agonists.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/23724987
Author(s): Du AL, Ji TL, Yang B, Cao JF, Zhang XG, et al.
Author(s): An J, Tzagarakis-Foster C, Scharschmidt TC, Lomri N, Leitman DC
Author(s): Jayakumar AR, Rao KV, Panickar KS, Moriyama M, Reddy PV, et al.
Author(s): Gibson CL, Gray LJ, Murphy SP, Bath PM
Author(s): Jover T, Tanaka H, Calderone A, Oguro K, Bennett MV, et al.
Author(s): Khaksari M, Soltani Z, Shahrokhi N, Moshtaghi G and Asadikaram G
Author(s): Shahrokhi N, Khaksari M, Soltani Z, Mahmoodi M, Nakhaee N
Author(s): Brass LM
Author(s): Wassertheil-Smoller S, Hendrix SL, Limacher M, Heiss G, Kooperberg C, et al.
Author(s): Cassidy A
Author(s): Michel T, Halabalaki M, Skaltsounis AL
Author(s): Rietjens IM, Sotoca AM, Vervoort J, Louisse J
Author(s): Paterni I, Granchi C1, Katzenellenbogen JA2, Minutolo F3
Author(s): Kronenberg F, Fugh-Berman A
Author(s): Lund TD, West TW, Tian LY, Bu LH, Simmons DL, et al.
Author(s): Bu L, Lephart ED
Author(s): Trieu VN, Uckun FM
Author(s): Setchell KD
Author(s): Schreihofer DA, Do KD, Schreihofer AM
Author(s): Burguete MC, Torregrosa PA, Asensio G, Fernando J, CastellA, et al.
Author(s): Bourque M, Dluzen DE, Di Paolo T
Author(s): Chiang SS, Pan TM
Author(s): Newton KM, Buist DS, Keenan NL, Anderson LA, LaCroix AZ
Author(s): Lapchak PA, Zhang JH and Noble-Haeusslein LJ
Author(s): Khaksari M, Mahmmodi R, Shahrokhi N, Shabani M, Joukar S, et al.
Author(s): Consiglio AR, Lucion AB