Author(s): Svoboda K, Block SM
We measured the force-velocity curves of single kinesin molecules attached to silica beads moving in an in vitro motility assay. Optical trapping interferometry was used to track movement with subnanometer precision and to apply calibrated, pN-sized forces to the beads. Velocity decreased linearly with increasing force, and kinesin molecules moved against applied loads of up to 5-6 pN. Comparison of force-velocity curves at limiting and saturating ATP concentrations suggests that the load-dependent diminution in kinesin velocity may be due to a decrease in the net displacement per molecule of ATP hydrolyzed, not simply to a slowing of the ATP turnover rate; kinesin would therefore appear to be a loosely coupled motor.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/8205624
Author(s): Rogers M, Serban D, Gyuris T, Scott M, Torchia T, et al.
Author(s): Pan KM, Stahl N, Prusiner SB
Author(s): Taraboulos A, Scott M, Semenov A, Avrahami D, Laszlo L, et al.
Author(s): Shyng SL, Moulder KL, Lesko A, Harris DA
Author(s): Nunziante M, Gilch S, Schätzl HM
Author(s): Lee KS, Magalhães AC, Zanata SM, Brentani RR, Martins VR, et al.
Author(s): Magalhães AC, Silva JA, Lee KS, Martins VR, Prado VF, et al.
Author(s): Negro A, Ballarin C, Bertoli A, Massimino ML, Sorgato MC
Author(s): Lorenz H, Windl O, Kretzschmar HA
Author(s): Ivanova L, Barmada S, Kummer T, Harris DA
Author(s): Butler DA, Scott MR, Bockman JM, Borchelt DR, Taraboulos A, et al.
Author(s): Pollard TD
Author(s): Howard J, Hyman AA
Author(s): Hachiya NS, Watanabe K, Yamada M, Sakasegawa Y, Kaneko K
Author(s): Brown DR
Author(s): Collinge J, Whittington MA, Sidle KC, Smith CJ, Palmer MS, et al.
Author(s): Brown DR, Qin K, Herms JW, Madlung A, Manson J, et al.
Author(s): Santuccione A, Sytnyk V, Leshchyns'ka I, Schachner M
Author(s): Hirokawa N, Takemura R
Author(s): Hirokawa N
Author(s): Peretti D, Peris L, Rosso S, Quiroga S, Cáceres A
Author(s): Setou M, Seog DH, Tanaka Y, Kanai Y, Takei Y, et al.
Author(s): Nakata T, Hirokawa N
Author(s): Mallik R, Gross SP
Author(s): Howard J, Hudspeth AJ, Vale RD
Author(s): Adachi N, Kohara K, Tsumoto T