Author(s): Schwanhausser B, Busse D, Li N, Dittmar G, Schuchhardt J, et al.
Gene expression is a multistep process that involves the transcription, translation and turnover of messenger RNAs and proteins. Although it is one of the most fundamental processes of life, the entire cascade has never been quantified on a genome-wide scale. Here we simultaneously measured absolute mRNA and protein abundance and turnover by parallel metabolic pulse labelling for more than 5,000 genes in mammalian cells. Whereas mRNA and protein levels correlated better than previously thought, corresponding half-lives showed no correlation. Using a quantitative model we have obtained the first genome-scale prediction of synthesis rates of mRNAs and proteins. We find that the cellular abundance of proteins is predominantly controlled at the level of translation. Genes with similar combinations of mRNA and protein stability shared functional properties, indicating that half-lives evolved under energetic and dynamic constraints. Quantitative information about all stages of gene expression provides a rich resource and helps to provide a greater understanding of the underlying design principles.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/21593866
Author(s): Kassell NF, Sasaki T, Colohan AR, Nazar G
Author(s): Stanton LW, Garrard LJ, Damm D, Garrick BL, Lam A, et al.
Author(s): Shi C, Awad IA, Jafari N, Lin S, Du P, et al.
Author(s): Pera J, Korostynski M, Krzyszkowski T, Czopek J, Slowik A, et al.
Author(s): Bolstad BM, Irizarry RA, Astrand M, Speed TP
Author(s): Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, et al.
Author(s): Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, et al.
Author(s): Onda H, Kasuya H, Takakura K, Hori T, Imaizumi T, et al.
Author(s): Suzuki H, Kanamaru K, Tsunoda H, Inada H, Kuroki M, et al.
Author(s): Macdonald RL, Zhang ZD, Ono S, Komuro T
Author(s): Vikman P, Beg S, Khurana TS, Hansen-Schwartz J, Edvinsson L
Author(s): Sasahara A, Kasuya H, Krischek B, Tajima A, Onda H, et al.
Author(s): Kikkawa Y, Matsuo S, Kameda K, Hirano M, Nakamizo A, et al.
Author(s): Borel CO, McKee A, Parra A, Haglund MM, Solan A, et al.
Author(s): Osuka K, Watanabe Y, Usuda N, Atsuzawa K, Wakabayashi T, et al.
Author(s): Yamaguchi-Okada M, Nishizawa S, Koide M, Nonaka Y
Author(s): Tang WH, Zhu G, Zhang JH, Chen Z, Liu Z, et al.
Author(s): Hughes JT, Schianchi PM
Author(s): Zhang J, Lewis A, Bernanke D, Zubkov A, Clower B
Author(s): Kim GH, Kellner CP, Hahn DK, Desantis BM, Musabbir M, et al.
Author(s): Zhou ML, Wu W, Ding YS, Zhang FF, Hang CH, et al.
Author(s): Wu Y, Tang K, Huang RQ, Zhuang Z, Cheng HL, et al.
Author(s): Suzuki H, Hasegawa Y, Kanamaru K, Zhang JH
Author(s): Meller R, Stevens SL, Minami M, Cameron JA, King S, et al.
Author(s): Xie Y, Sakatsume M, Nishi S, Narita I, Arakawa M, et al.
Author(s): Nagata K, Sasaki T, Mori T, Nikaido H, Kobayashi E, et al.
Author(s): Zubkov AY, Ogihara K, Bernanke DH, Parent AD, Zhang J
Author(s): Zhou C, Yamaguchi M, Colohan AR, Zhang JH
Author(s): Zubkov AY, Tibbs RE, Clower B, Ogihara K, Aoki K, et al.
Author(s): Sasaki T, Kassell NF, Yamashita M, Fujiwara S, Zuccarello M
Author(s): Sasaki T, Kassell NF, Zuccarello M, Nakagomi T, Fijiwara S, et al.
Author(s): Cahill J, Calvert JW, Solaroglu I, Zhang JH
Author(s): Sehba FA, Pluta RM, Zhang JH
Author(s): Ostergaard JR, Kristensen BO, Svehag SE, Teisner B, Miletic T
Author(s): Yanamoto H, Kikuchi H, Okamoto S, Nozaki K