Author(s): Macdonald RL, Zhang ZD, Ono S, Komuro T
Objective:Complementary deoxyribonucleic acid array analysis was used to determine whether vasospasm after subarachnoid hemorrhage (SAH) is associated with changes in gene expression.
Methods:Right SAHs were created in three monkeys, and the right and left middle cerebral arteries were collected 3, 7, or 14 days after SAH. Vasospasm was assessed by angiography performed on Day 0 and at tissue harvest. A complementary deoxyribonucleic acid array containing 5184 genes was used to screen for changes in gene expression by comparing the right and left middle cerebral arteries.
Results:There was significant expression (greater than fivefold expression of messenger ribonucleic acid compared with internal standard control) of 537 genes (10%) in the middle cerebral arteries. One hundred sixty-four genes (31%) did not change significantly, and 373 (69%) were differentially expressed at 3, 7, or 14 days after SAH. These 373 genes changed from 1.2- to 7-fold as compared with control arteries. The most common pattern was a progressive increase with increased time after SAH. The functions of differentially expressed genes included the regulation of gene expression, cell proliferation, inflammation, membrane proteins and receptors, kinases, and phosphatases. There was a marked increase in parathyroid hormone and parathyroid hormone receptor with time after SAH. Immunoblotting demonstrated a significant increase in parathyroid hormone receptor protein.
Conclusion:The up-regulation of these proteins involved in vascular relaxation suggests that they may play a role in vasospasm. The progressive increase in messenger ribonucleic acids involved in the functions noted suggests that the pathogenesis of cerebral vasospasm involves cell proliferation, inflammation, and possibly smooth muscle phenotype change.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/11950412
Author(s): Kassell NF, Sasaki T, Colohan AR, Nazar G
Author(s): Stanton LW, Garrard LJ, Damm D, Garrick BL, Lam A, et al.
Author(s): Shi C, Awad IA, Jafari N, Lin S, Du P, et al.
Author(s): Pera J, Korostynski M, Krzyszkowski T, Czopek J, Slowik A, et al.
Author(s): Bolstad BM, Irizarry RA, Astrand M, Speed TP
Author(s): Gentleman RC, Carey VJ, Bates DM, Bolstad B, Dettling M, et al.
Author(s): Saeed AI, Bhagabati NK, Braisted JC, Liang W, Sharov V, et al.
Author(s): Onda H, Kasuya H, Takakura K, Hori T, Imaizumi T, et al.
Author(s): Suzuki H, Kanamaru K, Tsunoda H, Inada H, Kuroki M, et al.
Author(s): Vikman P, Beg S, Khurana TS, Hansen-Schwartz J, Edvinsson L
Author(s): Sasahara A, Kasuya H, Krischek B, Tajima A, Onda H, et al.
Author(s): Kikkawa Y, Matsuo S, Kameda K, Hirano M, Nakamizo A, et al.
Author(s): Schwanhausser B, Busse D, Li N, Dittmar G, Schuchhardt J, et al.
Author(s): Borel CO, McKee A, Parra A, Haglund MM, Solan A, et al.
Author(s): Osuka K, Watanabe Y, Usuda N, Atsuzawa K, Wakabayashi T, et al.
Author(s): Yamaguchi-Okada M, Nishizawa S, Koide M, Nonaka Y
Author(s): Tang WH, Zhu G, Zhang JH, Chen Z, Liu Z, et al.
Author(s): Hughes JT, Schianchi PM
Author(s): Zhang J, Lewis A, Bernanke D, Zubkov A, Clower B
Author(s): Kim GH, Kellner CP, Hahn DK, Desantis BM, Musabbir M, et al.
Author(s): Zhou ML, Wu W, Ding YS, Zhang FF, Hang CH, et al.
Author(s): Wu Y, Tang K, Huang RQ, Zhuang Z, Cheng HL, et al.
Author(s): Suzuki H, Hasegawa Y, Kanamaru K, Zhang JH
Author(s): Meller R, Stevens SL, Minami M, Cameron JA, King S, et al.
Author(s): Xie Y, Sakatsume M, Nishi S, Narita I, Arakawa M, et al.
Author(s): Nagata K, Sasaki T, Mori T, Nikaido H, Kobayashi E, et al.
Author(s): Zubkov AY, Ogihara K, Bernanke DH, Parent AD, Zhang J
Author(s): Zhou C, Yamaguchi M, Colohan AR, Zhang JH
Author(s): Zubkov AY, Tibbs RE, Clower B, Ogihara K, Aoki K, et al.
Author(s): Sasaki T, Kassell NF, Yamashita M, Fujiwara S, Zuccarello M
Author(s): Sasaki T, Kassell NF, Zuccarello M, Nakagomi T, Fijiwara S, et al.
Author(s): Cahill J, Calvert JW, Solaroglu I, Zhang JH
Author(s): Sehba FA, Pluta RM, Zhang JH
Author(s): Ostergaard JR, Kristensen BO, Svehag SE, Teisner B, Miletic T
Author(s): Yanamoto H, Kikuchi H, Okamoto S, Nozaki K