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First harmful Dinophysis (Dinophyceae, Dinophysiales) bloom in the US is revealed by automated imaging flow cytometry

Author(s): Campbel L, Olson RJ, Sosik HM, Abraham A, Henrichs DW, et al.

Abstract

Imaging FlowCytobot (IFCB) combines video and flow cytometric technology to capture images of nano- and microplankton (∼10 to >100 μm) and to measure the chlorophyll fluorescence associated with each image. The images are of sufficient resolution to identify many organisms to genus or even species level. IFCB has provided >200 million images since its installation at the entrance to the Mission-Aransas estuary (Port Aransas, TX, USA) in September 2007. In early February 2008, Dinophysis cells (1–5 · mL−1) were detected by manual inspection of images; by late February, abundance estimates exceeded 200 cells · mL−1

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