Related Subjects

Fluorescence spectroscopy and multi-way techniques

Author(s): Murphy KR, Stedmon CA, Graeber D, Bro R


PARAllel FACtor analysis (PARAFAC) is increasingly used to decompose fluorescence excitation emission matrices (EEMs) into their underlying chemical components. In the ideal case where fluorescence conforms to Beers Law, this process can lead to the mathematical identification and quantification of independently varying fluorophores. However, many practical and analytical hurdles stand between EEM datasets and their chemical interpretation. This article provides a tutorial in the practical application of PARAFAC to fluorescence datasets, demonstrated using a dissolved organic matter (DOM) fluorescence dataset. A new toolbox for MATLAB is presented to support improved visualisation and sensitivity analyses of PARAFAC models in fluorescence spectroscopy.

Similar Articles

Estimating global "blue carbon" emissions from conversion and degradation of vegetated coastal ecosystems

Author(s): Pendleton L, Donato DC, Murray BC, Crooks S, Jenkins WA, et al.

Major role of marine vegetation on the oceanic carbon cycle

Author(s): Duarte CM, Middelburg JJ, Caraco N

Seagrass community metabolism: Assessing the carbon sink capacity of seagrass meadows

Author(s): Duarte CM, Marbà N, Gacia E, Fourqurean JW, Beggins J, et al.

Seagrass sediments as a global carbon sink: Isotopic constraints

Author(s): Kennedy H, Beggins J, Duarte CM, Fourqurean JW, Holmer M, et al.

Distribution of Terrestrially Derived Dissolved Organic Matter on the Southeastern U

Author(s): Moran MA, Pomeroy LR, Sheppard ES, Atkinson LP, Hodson RE

Limnol Oceanography 50: 686-697

Author(s): Stedmon CA, Markager S  (2005a) Resolving the variability in dissolved organic matter fluorescence in a temperate estuary and its catchment using PARAFAC analysis

Limnol Oceanography 50: 1415-1426

Author(s): Stedmon CA, Markager S (2005b) Tracing the production and degradation of autochthonous fractions of dissolved organic matter by fluorescence analysis