Clinical and preclinical translation of cell-based therapies using adipose tissue-derived cells

Author(s): Gimble JM, Guilak F,Bunnell BA

Abstract

Adipose tissue is now recognized as an accessible, abundant, and reliable site for the isolation of adult stem cells suitable for tissue engineering and regenerative medicine applications. The past decade has witnessed an explosion of preclinical data relating to the isolation, characterization, cryopreservation, differentiation, and transplantation of freshly isolated stromal vascular fraction cells and adherent, culture-expanded, adipose-derived stromal/stem cells in vitro and in animal models. This body of work has provided evidence supporting clinical translational applications of adipose-derived cells in safety and efficacy trials. The present article reviews the case reports and phase I-III clinical evidence using autologous adipose-derived cells that have been published, to date, in the fields of gastroenterology, neurology, orthopedics, reconstructive surgery, and related clinical disciplines. Future directions and challenges facing the field are discussed and evaluated.

Similar Articles

Non-expanded adipose stromal vascular fraction cell therapy for multiple sclerosis

Author(s): Riordan NH, Ichim TE, Min WP, Wang H, Solano F, et al.

Isolation and characterization of multi-potent stem cells from human orbital fat tissues

Author(s): Ho JH, Ma WH, Tseng TC, Chen YF, Chen MH, et al.

Isolation and characterization of canine adipose-derived mesenchymal stem cells

Author(s): Neupane M, Chang CC, Kiupel M,Yuzbasiyan-Gurkan V

Plasticity of human adipose lineage cells toward endothelial cells: physiological and therapeutic perspectives

Author(s): Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, et al.

Progenitor-enriched adipose tissue transplantation as rescue for breast implant complications

Author(s): Yoshimura K, Asano Y, Aoi N, Kurita M, Oshima Y, et al.

Autologous stem cells (adipose) and fibrin glue used to treat widespread traumatic calvarial defects: case report

Author(s): Lendeckel S, Jodicke A, Christophis P, Heidinger K, Wolff J, et al.

Adipose-derived stromal cells: Their identity and uses in clinical trials, an update

Author(s): Casteilla L, Planat-Benard V, Laharrague P,Cousin B

A simple modification of the separation method reduces heterogeneity of adipose-derived stem cells

Author(s): Griesche N, Luttmann W, Luttmann A, Stammermann T, Geiger H, et al.

Characterization of adipose tissue-derived cells isolated with the Celution system

Author(s): Lin K, Matsubara Y, Masuda Y, Togashi K, Ohno T, et al.

Sera of overweight people promote in vitro adipocyte differentiation of bone marrow stromal cells

Author(s): Di Bernardo G, Messina G, Capasso S, Del Gaudio S, Cipollaro M, et al.

Comparison of ex vivo expansion culture conditions of mesenchymal stem cells for human cell therapy

Author(s): Perez-Ilzarbe M, Diez-Campelo M, Aranda P, Tabera S, Lopez T, et al.

Inhibition of mesenchymal stromal cells by pre-activated lymphocytes and their culture media

Author(s): Valencic E, Loganes C, Cesana S, Piscianz E, Gaipa G, et al.

Ex vivo expansion of human mesenchymal stem cells in defined serum-free media

Author(s): Jung S, Panchalingam KM, Rosenberg L,Behie LA

Optimizing proliferation and characterization of multipotent stem cells from porcine adipose tissue

Author(s): Wang KH, Kao AP, Wangchen H, Wang FY, Chang CH, et al.

Intravenous administration of auto serum-expanded autologous mesenchymal stem cells in stroke

Author(s): Honmou O, Houkin K, Matsunaga T, Niitsu Y, Ishiai S, et al.

Expectations and strategies regarding islet transplantation: metabolic data from the GRAGIL 2 trial

Author(s): Badet L, Benhamou PY, Wojtusciszyn A, Baertschiger R, Milliat-Guittard L, et al.

Human CD34/CD90 ASCs are capable of growing as sphere clusters, producing high levels of VEGF and forming capillaries

Author(s): De Francesco F, Tirino V, Desiderio V, Ferraro G, D'Andrea F, et al.