Author(s): Cargnoni A, Piccinelli EC, Ressel L, Rossi D, Magatti M, et al.
Background and aims: We recently demonstrated that injection of conditioned medium (CM) generated from cells of the mesenchymal region of human amniotic membrane (AMTCs) reduces bleomycin-induced lung fibrosis in mice, suggesting a crucial role of paracrine factor(s) secreted by AMTCs in these beneficial effects. We further investigated this hypothesis, the mechanisms involved, the effects on some lung functional parameters and whether AMTC-secreted effector(s) are specific to these cells and not produced by other cell types, extending the time of analysis up to 28 days after treatment.
Methods: Bleomycin-challenged mice were either treated with AMTC-CM or CM generated from human skin fibroblasts, human peripheral blood mononuclear cells or Jurkat cells, or were left untreated. Mouse lungs were analyzed for content of pro-inflammatory and pro-fibrotic molecules, presence of lymphocytes and macrophages and for fibrosis level (through histological semi-quantitative evaluation and quantitative measurement of collagen content). Arterial blood gas analysis was also performed.
Results: Up to 28 days after delivery, AMTC-CM-treated mice developed reduced lung fibrosis with respect to mice treated with other CM types. AMTC-CM-treated mice had comparatively better preservation of blood gas parameters and showed lower lung content of interleukin-6, tumor necrosis factor-α, macrophage inflammatory protein-1α, monocyte chemoattractant protein-1 and transforming growth factor-β associated with reduced lung macrophage levels.
Conclusions: AMTC-CM prevents lung fibrosis in bleomycin-challenged mice, improving survival and preserving lung functional parameters such as blood gas exchanges. The specificity of AMTC-CM action was indicated by the absence of fibrosis reduction when other CM types were used. Finally, we provide some insights into the possible mechanisms underlying AMTC-CM-mediated control of fibrosis.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/24094500
Author(s): Wynn TA, Ramalingam TR
Author(s): Sabry MM, Elkalawy SAE, Abo-Elnour RKE, Abd-El-Maksod DF
Author(s): Leung VYL, Aladin DMK, Lv F, Tam V, Sun Y, et al.
Author(s): Qi Y, Jiang D, Sindrilaru A, Stegemann A, Schatz S, et al.
Author(s): Kishore R, Verma SK, Mackie AR, Vaughan EE, Abramova TV, et al.
Author(s): Mias C, Lairez O, Trouche E, Roncalli J, Calise D, et al.
Author(s): Hemphill C, Stavoe K, Khalpey Z
Author(s): Zagoura DS, Roubelakis MG, Bitsika V, Trohatou O, Pappa KI, et al.
Author(s): Zhang DG, Jiang MY, Miao DS
Author(s): Fujimoto KL, Miki T, Liu LJ, Hashizume R, Strom SC, et al.
Author(s): Sedrakyan S, Da Sacco S, Milanesi A, Shiri L, Petrosyan A, et al.
Author(s): Garcia O, Carraro G, Turcatel G, Hall M, Sedrakyan S, et al.
Author(s): Sun D, Bu L, Liu C, Yin Z, Zhou X, et al.
Author(s): Wen ST, Chen W, Chen HL, Lai CW, Yen CC, et al.
Author(s): Baulier E, Favreau F, Le Corf A, Jayle C, Schneider F, et al.
Author(s): Lee WY, Wei HJ, Lin WW, Yeh YC, Hwang SM, et al.
Author(s): Grisafi D, Pozzobon M, Dedja A, Vanzo V, Tomanin R, et al.
Author(s): Moodley Y, Atienza D, Manuelpillai U, Samuel CS, Tchongue J, et al.
Author(s): Cargnoni A, Ressel L, Rossi D, Poli A, Arienti D, et al.
Author(s): Wynn TA
Author(s): Klingberg F, Hinz B, White ES
Author(s): Yoon BS, Moon JH, Jun EK, Kim J, Maeng I, et al.
Author(s): Vosdoganes P, Wallace EM, Chan ST, Acharya R, Moss TJM, et al.
Author(s): Hodge A, Lourensz D, Vaghjiani V, Nguyen H, Tchongue J, et al.
Author(s): Jun EK, Zhang Q, Yoon BS, Moon JH, Lee G, et al.
Author(s): Fong CY, Tam K, Cheyyatraivendran S, Gan SU, Gauthaman K, et al.
Author(s): Arno AI, Amini-Nik S, Blit PH, Al-Shebab M, Belo C, et al.
Author(s): Jeon YK, Jang YH, Yoo DR, Kim SN, Lee SK, et al.
Author(s): Kim WS, Park BS, Sung JH, Yang JM, Park SB, et al.
Author(s): Jung H, Kim HH, Lee DH, Hwang YS, Yang HC, et al.
Author(s): Ding J, Ma Z, Shankowsky HA, Medina A, Tredget EE
Author(s): Lee SH, Jin SY, Song JS, Seo KK, Cho KH
Author(s): Onishi S, Sumiyoshi H, Kitamura S, Nagaya N
Author(s): Salazar KD, Lankford SM, Brody AR
Author(s): Cho JW, Kang MC, Lee KS
Author(s): Leung A, Crombleholme TM, Keswani SG
Author(s): Hu MS, Rennert RC, McArdle A, Chung MT, Walmsley GG, et al.
Author(s): Hosper NA, Bank RA, van den Berg PP
Author(s): Przybyt E,Krenning G, Brinker MGL, Harmsen MC
Author(s): Masum MM, Boersema M, Bank RA
Author(s): Skardal A, Mack D, Kapetanovic E, Atala A, Jackson JD, et al.
Author(s): Shi HX, Lin C, Lin BB, Wang ZG, Zhang HY, et al.
Author(s): Kinoshita T, Ishikawa Y, Arita M, Akishima-Fukasawa Y, Fujita K, et al.
Author(s): Desai VD, Hsia HC, Schwatzbauer JE
Author(s): Murad S, Grove D, Lindberg KA, Reynolds G, Sivarajah A, et al.
Author(s): Pinnell SR
Author(s): Tsutsumi K, Fujikawa H, Kaijkawa T, Takedachi M, Yamamoto T, et al.
Author(s): Myllyharju J
Author(s): Ananthanarayanan VS
Author(s): Li Y, Schellhorn HE
Author(s): van der Slot AJ, Zuurmond A, Bardoel AFJ, Wijmenga C, Pruijs HEH, et al.
Author(s): Pornprasertsuk S, Duarte WR, Mochida Y, Yamauchi M
Author(s): van der Slot AJ, van Dura EA, Attema J, Blauw B, DeGroot J, et al.
Author(s): Mast BA, Diegelmann RF, Krummel TM, Cohen IK
Author(s): Longaker MT, Whitby DJ, Ferguson MWJ, Lorenz HP, Harrison MR, et al.