Author(s): Honmou O, Houkin K, Matsunaga T, Niitsu Y, Ishiai S, et al.
Transplantation of human mesenchymal stem cells has been shown to reduce infarct size and improve functional outcome in animal models of stroke. Here, we report a study designed to assess feasibility and safety of transplantation of autologous human mesenchymal stem cells expanded in autologous human serum in stroke patients. We report an unblinded study on 12 patients with ischaemic grey matter, white matter and mixed lesions, in contrast to a prior study on autologous mesenchymal stem cells expanded in foetal calf serum that focused on grey matter lesions. Cells cultured in human serum expanded more rapidly than in foetal calf serum, reducing cell preparation time and risk of transmissible disorders such as bovine spongiform encephalomyelitis. Autologous mesenchymal stem cells were delivered intravenously 36-133 days post-stroke. All patients had magnetic resonance angiography to identify vascular lesions, and magnetic resonance imaging prior to cell infusion and at intervals up to 1 year after. Magnetic resonance perfusion-imaging and 3D-tractography were carried out in some patients. Neurological status was scored using the National Institutes of Health Stroke Scale and modified Rankin scores. We did not observe any central nervous system tumours, abnormal cell growths or neurological deterioration, and there was no evidence for venous thromboembolism, systemic malignancy or systemic infection in any of the patients following stem cell infusion. The median daily rate of National Institutes of Health Stroke Scale change was 0.36 during the first week post-infusion, compared with a median daily rate of change of 0.04 from the first day of testing to immediately before infusion. Daily rates of change in National Institutes of Health Stroke Scale scores during longer post-infusion intervals that more closely matched the interval between initial scoring and cell infusion also showed an increase following cell infusion. Mean lesion volume as assessed by magnetic resonance imaging was reduced by >20% at 1 week post-cell infusion. While we would emphasize that the current study was unblinded, did not assess overall function or relative functional importance of different types of deficits, and does not exclude placebo effects or a contribution of recovery as a result of the natural history of stroke, our observations provide evidence supporting the feasibility and safety of delivery of a relatively large dose of autologous mesenchymal human stem cells, cultured in autologous human serum, into human subjects with stroke and support the need for additional blinded, placebo-controlled studies on autologous mesenchymal human stem cell infusion in stroke.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/21493695
Author(s): Riordan NH, Ichim TE, Min WP, Wang H, Solano F, et al.
Author(s): Faustini M, Bucco M, Chlapanidas T, Lucconi G, Marazzi M, et al.
Author(s): Ho JH, Ma WH, Tseng TC, Chen YF, Chen MH, et al.
Author(s): Neupane M, Chang CC, Kiupel M,Yuzbasiyan-Gurkan V
Author(s): Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, et al.
Author(s): Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Inoue K, et al.
Author(s): Yoshimura K, Asano Y, Aoi N, Kurita M, Oshima Y, et al.
Author(s): Kim M, Kim I, Lee SK, Bang SI,Lim SY
Author(s): Lendeckel S, Jodicke A, Christophis P, Heidinger K, Wolff J, et al.
Author(s): Gimble JM, Guilak F,Bunnell BA
Author(s): Casteilla L, Planat-Benard V, Laharrague P,Cousin B
Author(s): Griesche N, Luttmann W, Luttmann A, Stammermann T, Geiger H, et al.
Author(s): Zhang S, Espandar L, Imhof KM,Bunnell BA
Author(s): Lin KJ, Loi MX, Lien GS, Cheng CF, Pao HY, et al.
Author(s): Niada S, Ferreira LM, Arrigoni E, Addis A, Campagnol M, et al.
Author(s): Farre-Guasch E, Marti-Page C, Hernadez-Alfaro F, Klein-Nulend J,Casals N
Author(s): Lin K, Matsubara Y, Masuda Y, Togashi K, Ohno T, et al.
Author(s): Spees JL, Gregory CA, Singh H, Tucker HA, Peister A, et al.
Author(s): Sundin M, Ringden O, Sundberg B, Nava S, Gotherstrom C, et al.
Author(s): Santos FD, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, et al.
Author(s): Di Bernardo G, Messina G, Capasso S, Del Gaudio S, Cipollaro M, et al.
Author(s): Perez-Ilzarbe M, Diez-Campelo M, Aranda P, Tabera S, Lopez T, et al.
Author(s): Valencic E, Loganes C, Cesana S, Piscianz E, Gaipa G, et al.
Author(s): Even MS, Sandusky CB,Barnard ND
Author(s): Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, et al.
Author(s): Felka T, Schafer R, De Zwart P,Aicher WK
Author(s): Hartmann I, Hollweck T, Haffner S, Krebs M, Meiser B, et al.
Author(s): Pal R, Hanwate M, Jan M,Totey S
Author(s): Rajaraman G, White J, Tan KS, Ulrich D, Rosamilia A, et al.
Author(s): Jung S, Panchalingam KM, Rosenberg L,Behie LA
Author(s): Chase LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, et al.
Author(s): Corotchi MC, Popa MA, Remes A, Sima LE, Gussi I, et al.
Author(s): Sun LY, Pang CY, Li DK, Liao CH, Huang WC, et al.
Author(s): Lin PC, Chen YL, Chiu SC, Yu YL, Chen SP, et al.
Author(s): Tsai LP, Lee KF, Fang JS,Liu IY
Author(s): Sun LY, Hsieh DK, Yu TC, Chiu HT, Lu SF, et al.
Author(s): Wang KH, Kao AP, Wangchen H, Wang FY, Chang CH, et al.
Author(s): Muller AM, Davenport M, Verrier S, Droeser R, Alini M, et al.
Author(s): Van Pham P, Bui KH, Ngo DQ, Vu NB, Truong NH, et al.
Author(s): Badet L, Benhamou PY, Wojtusciszyn A, Baertschiger R, Milliat-Guittard L, et al.
Author(s): Janssens S, Dubois C, Bogaert J, Theunissen K, Deroose C, et al.
Author(s): De Francesco F, Tirino V, Desiderio V, Ferraro G, D'Andrea F, et al.
Author(s): Traktuev DO, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.
Author(s): Lee HJ, Choi BH, Min BH,Park SR
Author(s): Kim WS, Park BS,Sung JH
Author(s): Park BS, Kim WS, Choi JS, Kim HK, Won JH, et al.