Author(s): Sundin M, Ringden O, Sundberg B, Nava S, Gotherstrom C, et al.
Background and objectives: Mesenchymal stromal cells (MSC) may be used in cellular therapy to treat graft-versus-host-disease and autoimmune disorders, and in regenerative medicine. Preliminary data suggest limited cellular allogeneic rejection, but less is known about humoral responses. The objective of this study was to investigate whether antibodies against MSC were present after hematopoietic stem cell transplantation (HSCT) including treatment with matched or mismatched allogeneic MSC.
Design and methods: Twelve patients were evaluated using flow cytometric cross matches (FCXM) and enzyme-linked immunosorbent assays. Expression of blood group antigens, regarded as alloantigens giving rise to humoral alloimmunity, on MSC were explored using flow cytometry and immunofluorescence.
Results: Three of 12 patients exhibited late positivity in the FCXM. In absorption studies, antibodies directed against fetal calf serum (FCS), a component of the MSC culture medium, were identified. Healthy individuals expressed varying levels of anti-FCS antibodies and the same pattern was seen in immunosuppressed HSCT patients. MSC did not express blood group antigens. The patients with positive FCXM are alive and well.
Interpretation and conclusions: We have shown that immunosuppressed patients can exhibit anti-FCS antibodies, but no alloantibodies, that may bind to MSC. These antibodies seem clinically insignificant.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/17666368
Author(s): Riordan NH, Ichim TE, Min WP, Wang H, Solano F, et al.
Author(s): Faustini M, Bucco M, Chlapanidas T, Lucconi G, Marazzi M, et al.
Author(s): Ho JH, Ma WH, Tseng TC, Chen YF, Chen MH, et al.
Author(s): Neupane M, Chang CC, Kiupel M,Yuzbasiyan-Gurkan V
Author(s): Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, et al.
Author(s): Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Inoue K, et al.
Author(s): Yoshimura K, Asano Y, Aoi N, Kurita M, Oshima Y, et al.
Author(s): Kim M, Kim I, Lee SK, Bang SI,Lim SY
Author(s): Lendeckel S, Jodicke A, Christophis P, Heidinger K, Wolff J, et al.
Author(s): Gimble JM, Guilak F,Bunnell BA
Author(s): Casteilla L, Planat-Benard V, Laharrague P,Cousin B
Author(s): Griesche N, Luttmann W, Luttmann A, Stammermann T, Geiger H, et al.
Author(s): Zhang S, Espandar L, Imhof KM,Bunnell BA
Author(s): Lin KJ, Loi MX, Lien GS, Cheng CF, Pao HY, et al.
Author(s): Niada S, Ferreira LM, Arrigoni E, Addis A, Campagnol M, et al.
Author(s): Farre-Guasch E, Marti-Page C, Hernadez-Alfaro F, Klein-Nulend J,Casals N
Author(s): Lin K, Matsubara Y, Masuda Y, Togashi K, Ohno T, et al.
Author(s): Spees JL, Gregory CA, Singh H, Tucker HA, Peister A, et al.
Author(s): Santos FD, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, et al.
Author(s): Di Bernardo G, Messina G, Capasso S, Del Gaudio S, Cipollaro M, et al.
Author(s): Perez-Ilzarbe M, Diez-Campelo M, Aranda P, Tabera S, Lopez T, et al.
Author(s): Valencic E, Loganes C, Cesana S, Piscianz E, Gaipa G, et al.
Author(s): Even MS, Sandusky CB,Barnard ND
Author(s): Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, et al.
Author(s): Felka T, Schafer R, De Zwart P,Aicher WK
Author(s): Hartmann I, Hollweck T, Haffner S, Krebs M, Meiser B, et al.
Author(s): Pal R, Hanwate M, Jan M,Totey S
Author(s): Rajaraman G, White J, Tan KS, Ulrich D, Rosamilia A, et al.
Author(s): Jung S, Panchalingam KM, Rosenberg L,Behie LA
Author(s): Chase LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, et al.
Author(s): Corotchi MC, Popa MA, Remes A, Sima LE, Gussi I, et al.
Author(s): Sun LY, Pang CY, Li DK, Liao CH, Huang WC, et al.
Author(s): Lin PC, Chen YL, Chiu SC, Yu YL, Chen SP, et al.
Author(s): Tsai LP, Lee KF, Fang JS,Liu IY
Author(s): Sun LY, Hsieh DK, Yu TC, Chiu HT, Lu SF, et al.
Author(s): Wang KH, Kao AP, Wangchen H, Wang FY, Chang CH, et al.
Author(s): Honmou O, Houkin K, Matsunaga T, Niitsu Y, Ishiai S, et al.
Author(s): Muller AM, Davenport M, Verrier S, Droeser R, Alini M, et al.
Author(s): Van Pham P, Bui KH, Ngo DQ, Vu NB, Truong NH, et al.
Author(s): Badet L, Benhamou PY, Wojtusciszyn A, Baertschiger R, Milliat-Guittard L, et al.
Author(s): Janssens S, Dubois C, Bogaert J, Theunissen K, Deroose C, et al.
Author(s): De Francesco F, Tirino V, Desiderio V, Ferraro G, D'Andrea F, et al.
Author(s): Traktuev DO, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.
Author(s): Lee HJ, Choi BH, Min BH,Park SR
Author(s): Kim WS, Park BS,Sung JH
Author(s): Park BS, Kim WS, Choi JS, Kim HK, Won JH, et al.
