Author(s): Niada S, Ferreira LM, Arrigoni E, Addis A, Campagnol M, et al.
Introduction: Adipose-derived stem cells (ASCs) are progenitor cells used in bone tissue engineering and regenerative medicine. Despite subcutaneous adipose tissue being more abundant, the buccal fat pad (BFP) is easily accessible for dentists and maxillofacial surgeons. For this reason, considering the need for preclinical study and the swine as an optimal animal model in tissue engineering applications, we compared the features of porcine ASCs (pASCs) from both tissue-harvesting sites.
Methods: ASCs were isolated from interscapular subcutaneous adipose tissue (ScI) and buccal fat pads of six swine. Cells were characterized for their stemness and multipotent features. Moreover, their osteogenic ability when cultured on titanium disks and silicon carbide-plasma-enhanced chemical vapor-deposition fragments, and their growth in the presence of autologous and heterologous serum were also assessed.
Results: Independent of the harvesting site, no differences in proliferation, viability, and clonogenicity were observed among all the pASC populations. Furthermore, when induced toward osteogenic differentiation, both ScI- and BFP-pASCs showed an increase of collagen and calcified extracellular matrix (ECM) production, alkaline phosphatase activity, and osteonectin expression, indicating their ability to differentiate toward osteoblast-like cells. In addition, they differentiated toward adipocyte-like cells, and chondrogenic induced pASCs were able to increase glycosaminoglycans (GAGs) production over time. When cells were osteoinduced on synthetic biomaterials, they significantly increased the amount of calcified ECM compared with control cells; moreover, titanium showed the osteoinductive effect on pASCs, also without chemical stimuli. Finally, these cells grew nicely in 10% FBS, and no benefits were produced by substitution with swine serum.
Conclusions: Swine buccal fat pad contains progenitor cells with mesenchymal features, and they also osteo-differentiate nicely in association with synthetic supports. We suggest that porcine BFP-ASCs may be applied in preclinical studies of periodontal and bone-defect regeneration.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/24330736
Author(s): Riordan NH, Ichim TE, Min WP, Wang H, Solano F, et al.
Author(s): Faustini M, Bucco M, Chlapanidas T, Lucconi G, Marazzi M, et al.
Author(s): Ho JH, Ma WH, Tseng TC, Chen YF, Chen MH, et al.
Author(s): Neupane M, Chang CC, Kiupel M,Yuzbasiyan-Gurkan V
Author(s): Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, et al.
Author(s): Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Inoue K, et al.
Author(s): Yoshimura K, Asano Y, Aoi N, Kurita M, Oshima Y, et al.
Author(s): Kim M, Kim I, Lee SK, Bang SI,Lim SY
Author(s): Lendeckel S, Jodicke A, Christophis P, Heidinger K, Wolff J, et al.
Author(s): Gimble JM, Guilak F,Bunnell BA
Author(s): Casteilla L, Planat-Benard V, Laharrague P,Cousin B
Author(s): Griesche N, Luttmann W, Luttmann A, Stammermann T, Geiger H, et al.
Author(s): Zhang S, Espandar L, Imhof KM,Bunnell BA
Author(s): Lin KJ, Loi MX, Lien GS, Cheng CF, Pao HY, et al.
Author(s): Farre-Guasch E, Marti-Page C, Hernadez-Alfaro F, Klein-Nulend J,Casals N
Author(s): Lin K, Matsubara Y, Masuda Y, Togashi K, Ohno T, et al.
Author(s): Spees JL, Gregory CA, Singh H, Tucker HA, Peister A, et al.
Author(s): Sundin M, Ringden O, Sundberg B, Nava S, Gotherstrom C, et al.
Author(s): Santos FD, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, et al.
Author(s): Di Bernardo G, Messina G, Capasso S, Del Gaudio S, Cipollaro M, et al.
Author(s): Perez-Ilzarbe M, Diez-Campelo M, Aranda P, Tabera S, Lopez T, et al.
Author(s): Valencic E, Loganes C, Cesana S, Piscianz E, Gaipa G, et al.
Author(s): Even MS, Sandusky CB,Barnard ND
Author(s): Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, et al.
Author(s): Felka T, Schafer R, De Zwart P,Aicher WK
Author(s): Hartmann I, Hollweck T, Haffner S, Krebs M, Meiser B, et al.
Author(s): Pal R, Hanwate M, Jan M,Totey S
Author(s): Rajaraman G, White J, Tan KS, Ulrich D, Rosamilia A, et al.
Author(s): Jung S, Panchalingam KM, Rosenberg L,Behie LA
Author(s): Chase LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, et al.
Author(s): Corotchi MC, Popa MA, Remes A, Sima LE, Gussi I, et al.
Author(s): Sun LY, Pang CY, Li DK, Liao CH, Huang WC, et al.
Author(s): Lin PC, Chen YL, Chiu SC, Yu YL, Chen SP, et al.
Author(s): Tsai LP, Lee KF, Fang JS,Liu IY
Author(s): Sun LY, Hsieh DK, Yu TC, Chiu HT, Lu SF, et al.
Author(s): Wang KH, Kao AP, Wangchen H, Wang FY, Chang CH, et al.
Author(s): Honmou O, Houkin K, Matsunaga T, Niitsu Y, Ishiai S, et al.
Author(s): Muller AM, Davenport M, Verrier S, Droeser R, Alini M, et al.
Author(s): Van Pham P, Bui KH, Ngo DQ, Vu NB, Truong NH, et al.
Author(s): Badet L, Benhamou PY, Wojtusciszyn A, Baertschiger R, Milliat-Guittard L, et al.
Author(s): Janssens S, Dubois C, Bogaert J, Theunissen K, Deroose C, et al.
Author(s): De Francesco F, Tirino V, Desiderio V, Ferraro G, D'Andrea F, et al.
Author(s): Traktuev DO, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.
Author(s): Lee HJ, Choi BH, Min BH,Park SR
Author(s): Kim WS, Park BS,Sung JH
Author(s): Park BS, Kim WS, Choi JS, Kim HK, Won JH, et al.
