Author(s): Even MS, Sandusky CB,Barnard ND
Despite considerable progress in the development of cell culture techniques, including the development of the serum- and protein-free media that now routinely support hybridoma and mammalian cell growth, fetal bovine serum (FBS) supplemented media are still commonly used: a practice that raises ethical, scientific and safety concerns. The use of FBS in hybridoma culture media is examined here, with regards to the development and production of monoclonal antibodies (mAbs), and it is our recommendation that researchers adopt serum-free cell culture methods to reduce animal use in this area.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/16427150
Author(s): Riordan NH, Ichim TE, Min WP, Wang H, Solano F, et al.
Author(s): Faustini M, Bucco M, Chlapanidas T, Lucconi G, Marazzi M, et al.
Author(s): Ho JH, Ma WH, Tseng TC, Chen YF, Chen MH, et al.
Author(s): Neupane M, Chang CC, Kiupel M,Yuzbasiyan-Gurkan V
Author(s): Planat-Benard V, Silvestre JS, Cousin B, Andre M, Nibbelink M, et al.
Author(s): Matsumoto D, Sato K, Gonda K, Takaki Y, Shigeura T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Hirohi T, et al.
Author(s): Yoshimura K, Sato K, Aoi N, Kurita M, Inoue K, et al.
Author(s): Yoshimura K, Asano Y, Aoi N, Kurita M, Oshima Y, et al.
Author(s): Kim M, Kim I, Lee SK, Bang SI,Lim SY
Author(s): Lendeckel S, Jodicke A, Christophis P, Heidinger K, Wolff J, et al.
Author(s): Gimble JM, Guilak F,Bunnell BA
Author(s): Casteilla L, Planat-Benard V, Laharrague P,Cousin B
Author(s): Griesche N, Luttmann W, Luttmann A, Stammermann T, Geiger H, et al.
Author(s): Zhang S, Espandar L, Imhof KM,Bunnell BA
Author(s): Lin KJ, Loi MX, Lien GS, Cheng CF, Pao HY, et al.
Author(s): Niada S, Ferreira LM, Arrigoni E, Addis A, Campagnol M, et al.
Author(s): Farre-Guasch E, Marti-Page C, Hernadez-Alfaro F, Klein-Nulend J,Casals N
Author(s): Lin K, Matsubara Y, Masuda Y, Togashi K, Ohno T, et al.
Author(s): Spees JL, Gregory CA, Singh H, Tucker HA, Peister A, et al.
Author(s): Sundin M, Ringden O, Sundberg B, Nava S, Gotherstrom C, et al.
Author(s): Santos FD, Andrade PZ, Abecasis MM, Gimble JM, Chase LG, et al.
Author(s): Di Bernardo G, Messina G, Capasso S, Del Gaudio S, Cipollaro M, et al.
Author(s): Perez-Ilzarbe M, Diez-Campelo M, Aranda P, Tabera S, Lopez T, et al.
Author(s): Valencic E, Loganes C, Cesana S, Piscianz E, Gaipa G, et al.
Author(s): Lindroos B, Boucher S, Chase L, Kuokkanen H, Huhtala H, et al.
Author(s): Felka T, Schafer R, De Zwart P,Aicher WK
Author(s): Hartmann I, Hollweck T, Haffner S, Krebs M, Meiser B, et al.
Author(s): Pal R, Hanwate M, Jan M,Totey S
Author(s): Rajaraman G, White J, Tan KS, Ulrich D, Rosamilia A, et al.
Author(s): Jung S, Panchalingam KM, Rosenberg L,Behie LA
Author(s): Chase LG, Yang S, Zachar V, Yang Z, Lakshmipathy U, et al.
Author(s): Corotchi MC, Popa MA, Remes A, Sima LE, Gussi I, et al.
Author(s): Sun LY, Pang CY, Li DK, Liao CH, Huang WC, et al.
Author(s): Lin PC, Chen YL, Chiu SC, Yu YL, Chen SP, et al.
Author(s): Tsai LP, Lee KF, Fang JS,Liu IY
Author(s): Sun LY, Hsieh DK, Yu TC, Chiu HT, Lu SF, et al.
Author(s): Wang KH, Kao AP, Wangchen H, Wang FY, Chang CH, et al.
Author(s): Honmou O, Houkin K, Matsunaga T, Niitsu Y, Ishiai S, et al.
Author(s): Muller AM, Davenport M, Verrier S, Droeser R, Alini M, et al.
Author(s): Van Pham P, Bui KH, Ngo DQ, Vu NB, Truong NH, et al.
Author(s): Badet L, Benhamou PY, Wojtusciszyn A, Baertschiger R, Milliat-Guittard L, et al.
Author(s): Janssens S, Dubois C, Bogaert J, Theunissen K, Deroose C, et al.
Author(s): De Francesco F, Tirino V, Desiderio V, Ferraro G, D'Andrea F, et al.
Author(s): Traktuev DO, Merfeld-Clauss S, Li J, Kolonin M, Arap W, et al.
Author(s): Lee HJ, Choi BH, Min BH,Park SR
Author(s): Kim WS, Park BS,Sung JH
Author(s): Park BS, Kim WS, Choi JS, Kim HK, Won JH, et al.
