Author(s): Hodge A, Lourensz D, Vaghjiani V, Nguyen H, Tchongue J, et al.
Background: Intravenous infusion of human amniotic epithelial cells (hAECs) has been shown to ameliorate hepatic fibrosis in murine models. Hepatic stellate cells (HSCs) are the principal collagen-secreting cells in the liver. The aim of this study was to investigate whether factors secreted by hAECs and present in hAEC-conditioned medium (CM) have anti-fibrotic effects on activated human HSCs.
Methods: Human AECs were isolated from the placenta and cultured. Human hepatic stellate cells were exposed to hAEC CM to determine potential anti-fibrotic effects.
Results: HSCs treated for 48 h with hAEC CM displayed a significant reduction in the expression of the myofibroblast markers α-smooth muscle actin and platelet-derived growth factor. Expression of the pro-fibrotic cytokine transforming growth factor-β1 (TGF-β1) and intracellular collagen were reduced by 45% and 46%, respectively. Human AEC CM induced HSC apoptosis in 11.8% of treated cells and reduced HSC proliferation. Soluble human leukocyte antigen-G1, a hAEC-derived factor, significantly decreased TGF-β1 and collagen production in activated HSCs, although the effect on collagen production was less than that of hAEC CM. The reduction in collagen and TGF-B1 could not be attributed to PGE2, relaxin, IL-10, TGF-B3, FasL or TRAIL.
Conclusions: Human AEC CM treatment suppresses markers of activation, proliferation and fibrosis in human HSCs as well as inducing apoptosis and reducing proliferation. Human AEC CM treatment may be effective in ameliorating liver fibrosis and warrants further study.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/24642017
Author(s): Wynn TA, Ramalingam TR
Author(s): Sabry MM, Elkalawy SAE, Abo-Elnour RKE, Abd-El-Maksod DF
Author(s): Leung VYL, Aladin DMK, Lv F, Tam V, Sun Y, et al.
Author(s): Qi Y, Jiang D, Sindrilaru A, Stegemann A, Schatz S, et al.
Author(s): Kishore R, Verma SK, Mackie AR, Vaughan EE, Abramova TV, et al.
Author(s): Mias C, Lairez O, Trouche E, Roncalli J, Calise D, et al.
Author(s): Hemphill C, Stavoe K, Khalpey Z
Author(s): Zagoura DS, Roubelakis MG, Bitsika V, Trohatou O, Pappa KI, et al.
Author(s): Zhang DG, Jiang MY, Miao DS
Author(s): Fujimoto KL, Miki T, Liu LJ, Hashizume R, Strom SC, et al.
Author(s): Sedrakyan S, Da Sacco S, Milanesi A, Shiri L, Petrosyan A, et al.
Author(s): Garcia O, Carraro G, Turcatel G, Hall M, Sedrakyan S, et al.
Author(s): Sun D, Bu L, Liu C, Yin Z, Zhou X, et al.
Author(s): Wen ST, Chen W, Chen HL, Lai CW, Yen CC, et al.
Author(s): Baulier E, Favreau F, Le Corf A, Jayle C, Schneider F, et al.
Author(s): Lee WY, Wei HJ, Lin WW, Yeh YC, Hwang SM, et al.
Author(s): Grisafi D, Pozzobon M, Dedja A, Vanzo V, Tomanin R, et al.
Author(s): Moodley Y, Atienza D, Manuelpillai U, Samuel CS, Tchongue J, et al.
Author(s): Cargnoni A, Piccinelli EC, Ressel L, Rossi D, Magatti M, et al.
Author(s): Cargnoni A, Ressel L, Rossi D, Poli A, Arienti D, et al.
Author(s): Wynn TA
Author(s): Klingberg F, Hinz B, White ES
Author(s): Yoon BS, Moon JH, Jun EK, Kim J, Maeng I, et al.
Author(s): Vosdoganes P, Wallace EM, Chan ST, Acharya R, Moss TJM, et al.
Author(s): Jun EK, Zhang Q, Yoon BS, Moon JH, Lee G, et al.
Author(s): Fong CY, Tam K, Cheyyatraivendran S, Gan SU, Gauthaman K, et al.
Author(s): Arno AI, Amini-Nik S, Blit PH, Al-Shebab M, Belo C, et al.
Author(s): Jeon YK, Jang YH, Yoo DR, Kim SN, Lee SK, et al.
Author(s): Kim WS, Park BS, Sung JH, Yang JM, Park SB, et al.
Author(s): Jung H, Kim HH, Lee DH, Hwang YS, Yang HC, et al.
Author(s): Ding J, Ma Z, Shankowsky HA, Medina A, Tredget EE
Author(s): Lee SH, Jin SY, Song JS, Seo KK, Cho KH
Author(s): Onishi S, Sumiyoshi H, Kitamura S, Nagaya N
Author(s): Salazar KD, Lankford SM, Brody AR
Author(s): Cho JW, Kang MC, Lee KS
Author(s): Leung A, Crombleholme TM, Keswani SG
Author(s): Hu MS, Rennert RC, McArdle A, Chung MT, Walmsley GG, et al.
Author(s): Hosper NA, Bank RA, van den Berg PP
Author(s): Przybyt E,Krenning G, Brinker MGL, Harmsen MC
Author(s): Masum MM, Boersema M, Bank RA
Author(s): Skardal A, Mack D, Kapetanovic E, Atala A, Jackson JD, et al.
Author(s): Shi HX, Lin C, Lin BB, Wang ZG, Zhang HY, et al.
Author(s): Kinoshita T, Ishikawa Y, Arita M, Akishima-Fukasawa Y, Fujita K, et al.
Author(s): Desai VD, Hsia HC, Schwatzbauer JE
Author(s): Murad S, Grove D, Lindberg KA, Reynolds G, Sivarajah A, et al.
Author(s): Pinnell SR
Author(s): Tsutsumi K, Fujikawa H, Kaijkawa T, Takedachi M, Yamamoto T, et al.
Author(s): Myllyharju J
Author(s): Ananthanarayanan VS
Author(s): Li Y, Schellhorn HE
Author(s): van der Slot AJ, Zuurmond A, Bardoel AFJ, Wijmenga C, Pruijs HEH, et al.
Author(s): Pornprasertsuk S, Duarte WR, Mochida Y, Yamauchi M
Author(s): van der Slot AJ, van Dura EA, Attema J, Blauw B, DeGroot J, et al.
Author(s): Mast BA, Diegelmann RF, Krummel TM, Cohen IK
Author(s): Longaker MT, Whitby DJ, Ferguson MWJ, Lorenz HP, Harrison MR, et al.
