Author(s): Kawamoto A, Gwon HC, Iwaguro H, Yamaguchi JI, Uchida S, et al.
Background: We investigated the therapeutic potential of ex vivo expanded endothelial progenitor cells (EPCs) for myocardial neovascularization.
Methods and results: Peripheral blood mononuclear cells obtained from healthy human adults were cultured in EPC medium and harvested 7 days later. Myocardial ischemia was induced by ligating the left anterior descending coronary artery in male Hsd:RH-rnu (athymic nude) rats. A total of 10(6) EPCs labeled with 1,1'-dioctadecyl-1 to 3,3,3',3'-tetramethylindocarbocyanine perchlorate were injected intravenously 3 hours after the induction of myocardial ischemia. Seven days later, fluorescence-conjugated Bandeiraea simplicifolia lectin I was administered intravenously, and the rats were immediately killed. Fluorescence microscopy revealed that transplanted EPCs accumulated in the ischemic area and incorporated into foci of myocardial neovascularization. To determine the impact on left ventricular function, 5 rats (EPC group) were injected intravenously with 10(6) EPCs 3 hours after ischemia; 5 other rats (control group) received culture media. Echocardiography, performed just before and 28 days after ischemia, disclosed ventricular dimensions that were significantly smaller and fractional shortening that was significantly greater in the EPC group than in the control group by day 28. Regional wall motion was better preserved in the EPC group. After euthanization on day 28, necropsy examination disclosed that capillary density was significantly greater in the EPC group than in the control group. Moreover, the extent of left ventricular scarring was significantly less in rats receiving EPCs than in controls. Immunohistochemistry revealed capillaries that were positive for human-specific endothelial cells.
Conclusions: Ex vivo expanded EPCs incorporate into foci of myocardial neovascularization and have a favorable impact on the preservation of left ventricular function.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/11156872
Author(s): Davenport R, Dennis M
Author(s): Sudlow CL, Warlow CP
Author(s): Fagan SC, Morgenstern LB, Petitta A, Ward RE, Tilley BC, et al.
Author(s): Hess DC, Wang S, Hamilton W, Lee S, Pardue C, et al.
Author(s): Wang S, Gross H, Lee SB, Pardue C, Waller J, et al.
Author(s): Borlongan CV, Fournier C, Stahl CE, Yu G, Xu L, et al.
Author(s): Wang X, Rosell A, Lo EH
Author(s): Switzer JA, Hall C, Gross H, Waller J, Nichols FT, et al.
Author(s): Leira EC, Hess DC, Torner JC, Adams HP Jr
Author(s): Henninger N, Chowdhury N, Fisher M, Moonis M
Author(s): Wu O, Langhorne P
Author(s): Matsukawa N, Yasuhara T, Hara K, Xu L, Maki M, et al.
Author(s): Hill MD, Moy CS, Palesch YY, Martin R, Dillon CR, et al.
Author(s): Toda H, Takahashi J, Iwakami N, Kimura T, Hoki S, et al.
Author(s): Ferrari A, Ehler E, Nitsch RM, Götz J
Author(s): Borlongan CV, Tajima Y, Trojanowski JQ, Lee VMY, Sanberg PR
Author(s): Veizovic T, Beech JS, Stroemer RP, Watson WP, Hodges H
Author(s): Modo M, Stroemer RP, Tang E, Veizovic T, Sowniski P, et al.
Author(s): Englund U, Bjorklund A, Wictorin K, Lindvall O, Kokaia M
Author(s): Kopen GC, Prockop DJ, Phinney DG
Author(s): Chen J, Li Y, Chopp M
Author(s): Li Y, Chopp M, Chen J, Wang L, Gautam SC, et al.
Author(s): Woodbury D, Schwarz EJ, Prockop DJ, Black IB
Author(s): Muñoz-Elias G, Marcus AJ, Coyne TM, Woodbury D, Black IB
Author(s): Borlongan CV, Hadman M, Sanberg CD, Sanberg PR
Author(s): Eglitis MA, Mezey E
Author(s): Li Y, Chen J, Wang L, Lu M, Chopp M
Author(s): Chen J, Sanberg PR, Li Y, Wang L, Lu M, et al.
Author(s): Hess DC, Hill WD, Martin-Studdard A, Carroll J, Brailer J, et al.
Author(s): Willing AE, Lixian J, Milliken M, Poulos S, Zigova T, et al.
Author(s): Chen J, Zhang ZG, Li Y, Wang L, Xu YX, et al.
Author(s): Riess P, Zhang C, Saatman KE, Laurer HL, Longhi LG, et al.
Author(s): Zhao LR, Duan WM, Reyes M, Keene CD, Verfaillie CM, et al.
Author(s): Peled A, Kollet O, Ponomaryov T, Petit I, Franitza S, et al.
Author(s): Yamaguchi J, Kusano KF, Masuo O, Kawamoto A, Silver M, et al.
Author(s): Reyes M, Dudek A, Jahagirdar B, Koodie L, Marker PH, et al.
Author(s): Rajantie I, Ilmonen M, Alminaite A, Ozerdem U, Alitalo K, et al.
Author(s): Mahmood A, Lu D, Chopp M
Author(s): Suárez-Monteagudo C, Hernández-Ramírez P, Alvarez-González L, García-Maeso I, de la Cuétara-Bernal K, et al.
Author(s): Borlongan CV
Author(s): Feuerstein GZ, Zaleska MM, Krams M, Wang X, Day M, et al.
Author(s): Keene CD, Ortiz-Gonzalez XR, Jiang YH, Largaespada DA, Verfaillie CM, et al.
Author(s): Di Nicola M, Carlo-Stella C, Magni M, Milanesi M, Longoni PD, et al.
Author(s): Jorgensen C, Djouad F, Apparailly F, Noël D
Author(s): Le Blanc K, Tammik L, Sundberg B, Haynesworth SE, Ringdén O
Author(s): Aggarwal S, Pittenger MF
Author(s): Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC
Author(s): Le Blanc K, Rasmusson I, Sundberg B, Götherström C, Hassan M, et al.
Author(s): Reyes M, Verfaillie CM
Author(s): Ayata C, Ropper AH
Author(s): Brouns R, De Deyn PP
Author(s): Aoki T, Sumii T, Mori T, Wang XY, Lo EH
Author(s): Subramaniam S, Hill MD
Author(s): Chang CF, Lin SZ, Chiang YH, Morales M, Chou J, et al.
Author(s): Castillo J, Alvarez-Sabín J, Martínez-Vila E, Montaner J, Sobrino T, et al.
Author(s): Vila N, Castillo J, Dávalos A, Chamorro A
Author(s): Castillo J, Leira R
Author(s): Doyle KP, Simon RP, Stenzel-Poore MP
Author(s): Wang GJ, Deng HY, Maier CM, Sun GH, Yenari MA
Author(s): Tang Y, Xu H, Du X, Lit L, Walker W, et al.
Author(s): Carmeliet P
Author(s): Masuda H, Asahara T
Author(s): Shi Q, Rafii S, Wu MH, Wijelath ES, Yu C, et al.
Author(s): Bompais H, Chagraoui J, Canron X, Crisan M, Liu XH, et al.
Author(s): Pirro M, Schillaci G, Menecali C, Bagaglia F, Paltriccia R, et al.
Author(s): Umemura T, Soga J, Hidaka T, Takemoto H, Nakamura S, et al.
Author(s): Del Papa N, Quirici N, Soligo D, Scavullo C, Cortiana M, et al.
Author(s): Kuwana M, Okazaki Y, Yasuoka H, Kawakami Y, Ikeda Y
Author(s): Heiss C, Keymel S, Niesler U, Ziemann J, Kelm M, et al.
Author(s): Kondo T, Hayashi M, Takeshita K, Numaguchi Y, Kobayashi K, et al.
Author(s): Michaud SE, Dussault S, Haddad P, Groleau J, Rivard A
Author(s): Botta R, Gao E, Stassi G, Bonci D, Pelosi E, et al.
Author(s): Madeddu P, Emanueli C, Pelosi E, Salis MB, Cerio AM, et al.
Author(s): Rouhl RP, van Oostenbrugge RJ, Damoiseaux J, Tervaert JW, Lodder J
Author(s): Erbs S, Linke A, Adams V, Lenk K, Thiele H, et al.
Author(s): Li ZQ, Zhang M, Jing YZ, Zhang WW, Liu Y, et al.
Author(s): Meluzín J, Janousek S, Mayer J, Groch L, Hornácek I, et al.
Author(s): Meluzín J, Mayer J, Groch L, Janousek S, Hornácek I, et al.
Author(s): Mocini D, Staibano M, Mele L, Giannantoni P, Menichella G, et al.
Author(s): Perin EC, Dohmann HF, Borojevic R, Silva SA, Sousa AL, et al.
Author(s): Perin EC, Dohmann HF, Borojevic R, Silva SA, Sousa AL, et al.
Author(s): Strauer BE, Brehm M, Zeus T, Bartsch T, Schannwell C, et al.
Author(s): Strauer BE, Brehm M, Zeus T, Köstering M, Hernandez A, et al.
Author(s): Numaguchi Y, Sone T, Okumura K, Ishii M, Morita Y, et al.
Author(s): Döbert N, Britten M, Assmus B, Berner U, Menzel C, et al.
Author(s): Lev EI, Kleiman NS, Birnbaum Y, Harris D, Korbling M, et al.
Author(s): Hristov M, Heussen N, Schober A, Weber C
Author(s): Dimmeler S, Zeiher AM, Schneider MD
Author(s): Higashi Y, Kimura M, Hara K, Noma K, Jitsuiki D, et al.
Author(s): Tateishi-Yuyama E, Matsubara H, Murohara T, Ikeda U, Shintani S, et al.
Author(s): Taguchi A, Matsuyama T, Moriwaki H, Hayashi T, Hayashida K, et al.
Author(s): Ghani U, Shuaib A, Salam A, Nasir A, Shuaib U, et al.
