Author(s): Borlongan CV, Tajima Y, Trojanowski JQ, Lee VMY, Sanberg PR
This study was designed to explore the efficacy of a human clone cell line as an alternative neural graft source and to validate the practice of cryopreservation and xenografting as logistical approaches toward conducting neural transplantation. We investigated the biological effects of transplanting cultured human neurons (NT2N cells) derived from a well-characterized embryonal carcinoma cell line into the brains of rats subjected to transient, focal cerebral ischemia induced by embolic occlusion of the middle cerebral artery. At 1 month and extending throughout the 6-month posttransplantation test period, ischemic animals that were transplanted with NT2N cells and treated with an immunosuppressive drug displayed a significant improvement in a passive avoidance task as well as a normalization of asymmetrical motor behavior compared to ischemic animals that received rat fetal cerebellar cell grafts or vehicle alone. Remarkably, cryopreserved NT2N cell grafts compared with fresh NT2N cell grafts, remained viable in the immunosuppressed rat brain and effective in producing behavioral recovery in immunosuppressed ischemic animals. The long-term viability of cryopreserved NT2N cell xenografts in vivo and their sustained effectiveness in promoting behavioral recovery suggest potential utilization of xenografting and cryopreservation as useful protocols for establishing clone cell lines as graft source in neural transplantation therapies for central nervous system disorders.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/9500961
Author(s): Davenport R, Dennis M
Author(s): Sudlow CL, Warlow CP
Author(s): Fagan SC, Morgenstern LB, Petitta A, Ward RE, Tilley BC, et al.
Author(s): Hess DC, Wang S, Hamilton W, Lee S, Pardue C, et al.
Author(s): Wang S, Gross H, Lee SB, Pardue C, Waller J, et al.
Author(s): Borlongan CV, Fournier C, Stahl CE, Yu G, Xu L, et al.
Author(s): Wang X, Rosell A, Lo EH
Author(s): Switzer JA, Hall C, Gross H, Waller J, Nichols FT, et al.
Author(s): Leira EC, Hess DC, Torner JC, Adams HP Jr
Author(s): Henninger N, Chowdhury N, Fisher M, Moonis M
Author(s): Wu O, Langhorne P
Author(s): Matsukawa N, Yasuhara T, Hara K, Xu L, Maki M, et al.
Author(s): Hill MD, Moy CS, Palesch YY, Martin R, Dillon CR, et al.
Author(s): Toda H, Takahashi J, Iwakami N, Kimura T, Hoki S, et al.
Author(s): Ferrari A, Ehler E, Nitsch RM, Götz J
Author(s): Veizovic T, Beech JS, Stroemer RP, Watson WP, Hodges H
Author(s): Modo M, Stroemer RP, Tang E, Veizovic T, Sowniski P, et al.
Author(s): Englund U, Bjorklund A, Wictorin K, Lindvall O, Kokaia M
Author(s): Kopen GC, Prockop DJ, Phinney DG
Author(s): Chen J, Li Y, Chopp M
Author(s): Li Y, Chopp M, Chen J, Wang L, Gautam SC, et al.
Author(s): Woodbury D, Schwarz EJ, Prockop DJ, Black IB
Author(s): Muñoz-Elias G, Marcus AJ, Coyne TM, Woodbury D, Black IB
Author(s): Borlongan CV, Hadman M, Sanberg CD, Sanberg PR
Author(s): Eglitis MA, Mezey E
Author(s): Li Y, Chen J, Wang L, Lu M, Chopp M
Author(s): Chen J, Sanberg PR, Li Y, Wang L, Lu M, et al.
Author(s): Hess DC, Hill WD, Martin-Studdard A, Carroll J, Brailer J, et al.
Author(s): Willing AE, Lixian J, Milliken M, Poulos S, Zigova T, et al.
Author(s): Chen J, Zhang ZG, Li Y, Wang L, Xu YX, et al.
Author(s): Riess P, Zhang C, Saatman KE, Laurer HL, Longhi LG, et al.
Author(s): Zhao LR, Duan WM, Reyes M, Keene CD, Verfaillie CM, et al.
Author(s): Peled A, Kollet O, Ponomaryov T, Petit I, Franitza S, et al.
Author(s): Yamaguchi J, Kusano KF, Masuo O, Kawamoto A, Silver M, et al.
Author(s): Reyes M, Dudek A, Jahagirdar B, Koodie L, Marker PH, et al.
Author(s): Rajantie I, Ilmonen M, Alminaite A, Ozerdem U, Alitalo K, et al.
Author(s): Mahmood A, Lu D, Chopp M
Author(s): Suárez-Monteagudo C, Hernández-Ramírez P, Alvarez-González L, García-Maeso I, de la Cuétara-Bernal K, et al.
Author(s): Borlongan CV
Author(s): Feuerstein GZ, Zaleska MM, Krams M, Wang X, Day M, et al.
Author(s): Keene CD, Ortiz-Gonzalez XR, Jiang YH, Largaespada DA, Verfaillie CM, et al.
Author(s): Di Nicola M, Carlo-Stella C, Magni M, Milanesi M, Longoni PD, et al.
Author(s): Jorgensen C, Djouad F, Apparailly F, Noël D
Author(s): Le Blanc K, Tammik L, Sundberg B, Haynesworth SE, Ringdén O
Author(s): Aggarwal S, Pittenger MF
Author(s): Tse WT, Pendleton JD, Beyer WM, Egalka MC, Guinan EC
Author(s): Le Blanc K, Rasmusson I, Sundberg B, Götherström C, Hassan M, et al.
Author(s): Reyes M, Verfaillie CM
Author(s): Ayata C, Ropper AH
Author(s): Brouns R, De Deyn PP
Author(s): Aoki T, Sumii T, Mori T, Wang XY, Lo EH
Author(s): Subramaniam S, Hill MD
Author(s): Chang CF, Lin SZ, Chiang YH, Morales M, Chou J, et al.
Author(s): Castillo J, Alvarez-Sabín J, Martínez-Vila E, Montaner J, Sobrino T, et al.
Author(s): Vila N, Castillo J, Dávalos A, Chamorro A
Author(s): Castillo J, Leira R
Author(s): Doyle KP, Simon RP, Stenzel-Poore MP
Author(s): Wang GJ, Deng HY, Maier CM, Sun GH, Yenari MA
Author(s): Tang Y, Xu H, Du X, Lit L, Walker W, et al.
Author(s): Carmeliet P
Author(s): Masuda H, Asahara T
Author(s): Shi Q, Rafii S, Wu MH, Wijelath ES, Yu C, et al.
Author(s): Bompais H, Chagraoui J, Canron X, Crisan M, Liu XH, et al.
Author(s): Pirro M, Schillaci G, Menecali C, Bagaglia F, Paltriccia R, et al.
Author(s): Umemura T, Soga J, Hidaka T, Takemoto H, Nakamura S, et al.
Author(s): Del Papa N, Quirici N, Soligo D, Scavullo C, Cortiana M, et al.
Author(s): Kuwana M, Okazaki Y, Yasuoka H, Kawakami Y, Ikeda Y
Author(s): Heiss C, Keymel S, Niesler U, Ziemann J, Kelm M, et al.
Author(s): Kondo T, Hayashi M, Takeshita K, Numaguchi Y, Kobayashi K, et al.
Author(s): Michaud SE, Dussault S, Haddad P, Groleau J, Rivard A
Author(s): Botta R, Gao E, Stassi G, Bonci D, Pelosi E, et al.
Author(s): Kawamoto A, Gwon HC, Iwaguro H, Yamaguchi JI, Uchida S, et al.
Author(s): Madeddu P, Emanueli C, Pelosi E, Salis MB, Cerio AM, et al.
Author(s): Rouhl RP, van Oostenbrugge RJ, Damoiseaux J, Tervaert JW, Lodder J
Author(s): Erbs S, Linke A, Adams V, Lenk K, Thiele H, et al.
Author(s): Li ZQ, Zhang M, Jing YZ, Zhang WW, Liu Y, et al.
Author(s): Meluzín J, Janousek S, Mayer J, Groch L, Hornácek I, et al.
Author(s): Meluzín J, Mayer J, Groch L, Janousek S, Hornácek I, et al.
Author(s): Mocini D, Staibano M, Mele L, Giannantoni P, Menichella G, et al.
Author(s): Perin EC, Dohmann HF, Borojevic R, Silva SA, Sousa AL, et al.
Author(s): Perin EC, Dohmann HF, Borojevic R, Silva SA, Sousa AL, et al.
Author(s): Strauer BE, Brehm M, Zeus T, Bartsch T, Schannwell C, et al.
Author(s): Strauer BE, Brehm M, Zeus T, Köstering M, Hernandez A, et al.
Author(s): Numaguchi Y, Sone T, Okumura K, Ishii M, Morita Y, et al.
Author(s): Döbert N, Britten M, Assmus B, Berner U, Menzel C, et al.
Author(s): Lev EI, Kleiman NS, Birnbaum Y, Harris D, Korbling M, et al.
Author(s): Hristov M, Heussen N, Schober A, Weber C
Author(s): Dimmeler S, Zeiher AM, Schneider MD
Author(s): Higashi Y, Kimura M, Hara K, Noma K, Jitsuiki D, et al.
Author(s): Tateishi-Yuyama E, Matsubara H, Murohara T, Ikeda U, Shintani S, et al.
Author(s): Taguchi A, Matsuyama T, Moriwaki H, Hayashi T, Hayashida K, et al.
Author(s): Ghani U, Shuaib A, Salam A, Nasir A, Shuaib U, et al.
