A simple determination of steroidal alkaloid glycosides by thin-laer chromatography immunostaining using monoclonal antibody against solamargine

Author(s): Tanaka H, Putalun W, Tsuzaki C, Shoyama Y


A method of determination for solasodine glycosides by using thin-layer chromatography (TLC) immunostaining was investigated. Solasodine glycosides separated by silica gel TLC were transferred to a polyvinylidene difluoride membrane. The membrane was treated with sodium periodate solution followed by bovine serum albumin (BSA), resulting in a solasodine glycoside-BSA conjugate. Conjugation was confirmed by matrix-assisted laser desorption/ionization mass spectrometry. Individual spots were stained by monoclonal antibody against solamargine. Immunostaining of solasodine glycosides was more sensitive compared to other stainings. The newly established immunostaining method can be extended to analysis of the distribution of solasodine glycoside in the plant body.

Similar Articles

Effects of American ginseng berry extract on blood glucose levels in ob/ob mice

Author(s): Xie JT, Aung HH, Wu JA, Attele AS, Yuan CS

Chemical studies on crude drug processing

Author(s): Kitagawa I, Taniyama T, Shibuya H, Noda T, Yoshikawa M

High-performance liquid chromatography analysis of acidic saponins of ginseng and related plants

Author(s): Yamaguchi H, Kasai R, Matsuura H, Tanaka O, Fuwa T

New Flora of Korea

Author(s): Lee YN

Inhibitory lignans against NFAT transcription factor from Acanthopanax koreanum

Author(s): Cai XF, Lee IS, Dat NT, Shen G, Kang JS, et al.

Lupane glycosides from the leaves of Acanthopanax koreanum

Author(s): Choi HS, Kim HJ, Nam SG, Kim IS, Lee KT, et al.

Lupane triterpene glycosides from leaves of Acanthopanax koreanum and their cytotoxic activity

Author(s): Nhiem NX, Tung NH, Kiem PV, Minh CV, Ding Y, et al.

Studies on the chemical constituents of Kalopanax septemlobus

Author(s): Yao HK, Duan JY, Li Y, Wang JH, Yin XX, et al.

American ginseng suppresses colitis through p53-mediated apoptosis of inflammatory cells

Author(s): Jin Y, Hofseth AB, Cui XL, Windust AJ, Poudyal D, et al.