A Simple Isocratic HPLC Method for the Simultaneous Determination of Curcuminoids in Commercial Turmeric Extracts

Author(s): Wichitnithad W, Jongaroonngamsang N, Pummangura S, Rojsitthisak P

Abstract

Turmeric (Curcuma longa) extracts contain three curcuminoids (curcumin, desmethoxycurcumin and bisdesmethoxycurcumin) as major bioactive substances. Previously reported HPLC-UV methods for the determination of curcuminoids have several disadvantages, including unsatisfactory separation times, poor resolution and/or complicated solvent mixtures with gradient elution.

Objective

To develop a simple isocratic HPLC-UV method for the simultaneous determination of individual curcuminoids for the quality control of turmeric extracts.

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