Antioxidant and free radical-scavenging properties of ethanolic extracts of defatted borage (Borago offcinalis L

Author(s): Wettasinghe M, Shahidi F


Borage meal exerted a concentration-dependent antioxidant activity in a meat model system. At 2% (w/w), it inhibited (p≤0.05) 2-thiobarbituric acid-reactive substances (TBARS), hexanal and total volatile formation in meat by 26.5, 30.5 and 18.6%, respectively. Antioxidant compounds in the meal were concentrated at optimum extraction conditions (in 52% ethanol at 74°C for 62 min) predicted by response surface methodology (RSM). The resulting extract inhibited (p⩽0.05) the coupled oxidation of β-carotene and linoleate in a β-carotene-linoleate system. The system containing extract at a level providing 200 ppm phenolics retained 81% of the initial β-carotene after 2 h of assay whereas the control retained only 11%. Inhibition (p⩽0.05) of TBARS, hexanal and total volatile formation in a meat system containing 200 ppm extract ranged from 18.9 to 88.3%, depending upon the concentration being tested. The extract inhibited (p⩽0.05) conjugated diene, hexanal and total volatile formation in bulk corn oil (8.3–49.6% inhibition) and corn-oil-in water emulsion (5.2–32.2% inhibition). Hydrogen peroxide, hydroxyl radical and superoxide radical-scavenging properties of the extract were somewhat less than, but comparable to, those observed for trans-sinapic acid at similar concentrations of phenolics. At 200 ppm, a 100% quenching of the hydroxyl radical and superoxide radical was evident. The extract scavenged 29–75% of the hydrogen peroxide in assay media after 10 min of assay as compared to 3% reduction in the control.

Similar Articles

Variation in content of bioactive components in broccoli

Author(s): Jeffery EH, Brown AF, Kurilich AC, Keck AS, Matusheski N, et al.

Rocket "Eruca sativa": a salad herb with potential gastric anti-ulcer activity

Author(s): Alqasoumi S, Al-Sohaibani M, Al-Howiriny T, Al-Yahya M, Rafatullah S

Phytochemical analysis and antibacterial activity of Eruca sativa seed

Author(s): Gulfraz M, Sadiq A, Tariq H, Imran M, Qureshi R, Zeenat A

Anti-Inflammatory activity of Pandanus odoratissimus extract

Author(s): Londonkar R, Kamble A, Reddy VC

Phytochemicals Methods

Author(s): Harborne JB

Preliminary Phytochemical Analysis of some Plant Seeds

Author(s): Ajayi IA, Ajibade O, Oderinde RA

Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand

Author(s): Chanwitheesuk A, Teerawutgulrag A, Rakariyatham N

Polyphenolic Profile and Antioxidant Activities of Nigella sativa Seed Extracts in vitro and in vivo

Author(s): Meziti A, Meziti H, Boudiaf K, Mustapha B, Bouriche H

Determination of gallotannin with rhodanine

Author(s): Inoue KH, Hagerman AE

Hydrogen Peroxide radical scavenging and total antioxidant activity of hawthorn

Author(s): Keser S, Celik S, Turkoglu S, Yilmaz O, Turkoglu I

HPLC Analysis of Flavonoids in Eupatorium littorale

Author(s): De Oliveira BH, Nakashimab T, Filhoc JS, Frehsea FL

Phytochemical analysis and antibacterial activity of Eruca sativa seed

Author(s): Gulfraz M, Sadiq A, Tariq H, Imran M, Qureshi R, Zeenat A