Cis-9, trans-11-conjugated linoleic acid but not its precursor trans-vaccenic acid attenuate inflammatory markers in the human colonic epithelial cell line Caco-2

Author(s): Reynolds CM, Loscher CE, Moloney AP, Roche HM


Trans-vaccenic acid (TVA) is a natural trans fatty acid found in ruminant food produce. It is converted to the cis-9, trans-11 isomer of conjugated linoleic acid (c9, t11-CLA) by the action of stearoyl-CoA desaturase (SCD) in tissue. c9, t11-CLA has been associated with anti-inflammatory effects and also affects lipid metabolism. The aim of the present study was to determine if TVA is bioconverted to c9, t11-CLA in intestinal epithelial cells and to ascertain whether TVA has effects similar to c9, t11-CLA on markers of inflammation relevant to inflammatory bowel disease. The present study demonstrated that TVA treatment led to significant bioconversion into c9, t11-CLA in Caco-2 cells. Treatment with both TVA and c9, t11-CLA resulted in alteration of cellular fatty acid profile and SCD activity in the Caco-2 cell line. However, CLA, but not TVA, significantly modulated transcription of TNF-alpha, IL-12, IL-6 and production of IL-12 by these cells. Thus the present study established that TVA treatment can alter SCD desaturation indices and induce compositional changes in the fatty acid profile of the Caco-2 cell model of the human intestinal epithelium but this is not associated with functional effects on markers of the inflammatory response.

Similar Articles

Ten misconceptions about antioxidants

Author(s): Bast A, Haenen GR

Cellular stress responses, hormetic phytochemicals and vitagenes in aging and longevity

Author(s): Calabrese V, Cornelius C, Dinkova-Kostova AT, Iavicoli I, Di Paola R, et al.

Shifting from the single to the multitarget paradigm in drug discovery

Author(s): Medina-Franco JL, Giulianotti MA, Welmaker GS, Houghten RA

Amaranth: A Pseudo-cereal with nutraceutical properties

Author(s): Huerta-Ocampo JA, Barba de la Rosa AP

Peculiarities of amaranth oil influence on the liver antioxidant system and blood of mice with tumor growth

Author(s): Yelisyeyeva OP, Kamisnkyy DV, Cherkas AP, Ambarova LI, Vyshemyrska OR, et al.

The effect of Amaranth oil on monolayers of artificial lipids and hepatocyte plasma membranes with adrenalin-induced stress

Author(s): Yelisyeyeva OP, Semen KO, Ostrovska GV, Kaminskyy DV, Sirota TV, et al.

Effect of amaranth seeds in diet on oxidative status in plasma and selected tissues of high fructose-fed rats

Author(s): Pasko P, Bartn H, Zagrodizki P, Chlopicka J, Izewska A, et al.

Amaranth oil application for coronary heart disease and hypertension

Author(s): Martirosyan DM, Miroshnichenko LA, Kulakova SN, Pogojeva AV, Zoloedov VI

Nutrilipidomics: a tool for personalized health J

Author(s): Chatgilialoglu C, Ferreri C

Fatty acid profile of erythrocyte membranes as possible biomarker of longevity

Author(s): Puca AA, Andrew P, Novelli V, Anselmi CV, Somalvico F, et al.

Oxidative stress and antioxidants in interstitial lung disease

Author(s): Bast A, Weseler AR, Haenen GR, den Hartog GJ