Development and Validation of HPLC Method for Quantification of Phytoconstituents in Phyllanthus emblica

Author(s): Sawant L, Prabhakar B, Mahajan A, Pai N, Pandita N


high performance liquid chromatography method coupled with diode array detection was developed to simultaneously determine eight different marker compounds in Phyllanthus emblica. These markers are ascorbic acid (1), gallic acid (2), methyl gallate (3), chlorogenic acid (4), ethyl gallate (5), ellagic acid (6), rutin (7) and chebulagic acid (8). HPLC analysis was carried out at wavelength 272nm. The developed method was able to determine the marker compounds with excellent resolution, precision and recovery. The chromatographic separation was performed on Thermo Scientific BDS HYPERSIL Phenyl reversed-phase column (250mm×4.6mm, 5μm). The mobile phase was consisted of 0.1% ortho-phosphoric acid: Methanol (95:05v/v) (A) and acetonitrile (B) at a flow rate of 1.5 ml/min gradient mode. Regression equations showed good linear relationships (R2 > 0.998) between the peak area of each marker and concentration. The assay was reproducible with overall intra- and inter-day variation of less than 3.4%. The recoveries, measured at three concentration levels, varied from 97.8% to 101.1%. The method was applied to determine the amounts of the marker compounds in dried fruits of Phyllanthus emblica, and significant variations in phytoconstituents were observed.

Similar Articles

Variation in content of bioactive components in broccoli

Author(s): Jeffery EH, Brown AF, Kurilich AC, Keck AS, Matusheski N, et al.

Rocket "Eruca sativa": a salad herb with potential gastric anti-ulcer activity

Author(s): Alqasoumi S, Al-Sohaibani M, Al-Howiriny T, Al-Yahya M, Rafatullah S

Phytochemical analysis and antibacterial activity of Eruca sativa seed

Author(s): Gulfraz M, Sadiq A, Tariq H, Imran M, Qureshi R, Zeenat A

Anti-Inflammatory activity of Pandanus odoratissimus extract

Author(s): Londonkar R, Kamble A, Reddy VC

Phytochemicals Methods

Author(s): Harborne JB

Preliminary Phytochemical Analysis of some Plant Seeds

Author(s): Ajayi IA, Ajibade O, Oderinde RA

Screening of antioxidant activity and antioxidant compounds of some edible plants of Thailand

Author(s): Chanwitheesuk A, Teerawutgulrag A, Rakariyatham N

Polyphenolic Profile and Antioxidant Activities of Nigella sativa Seed Extracts in vitro and in vivo

Author(s): Meziti A, Meziti H, Boudiaf K, Mustapha B, Bouriche H

Determination of gallotannin with rhodanine

Author(s): Inoue KH, Hagerman AE

Hydrogen Peroxide radical scavenging and total antioxidant activity of hawthorn

Author(s): Keser S, Celik S, Turkoglu S, Yilmaz O, Turkoglu I

HPLC Analysis of Flavonoids in Eupatorium littorale

Author(s): De Oliveira BH, Nakashimab T, Filhoc JS, Frehsea FL

Phytochemical analysis and antibacterial activity of Eruca sativa seed

Author(s): Gulfraz M, Sadiq A, Tariq H, Imran M, Qureshi R, Zeenat A