Author(s): Ayres DC, Marcucci MC, Giorgio S
Leishmaniasis, an endemic parasitosis that leads to chronic cutaneous, mucocutaneous or visceral lesions, is part of those diseases, which still requires improved control tools. Propolis has shown activities against different bacteria, fungi, and parasites. In this study we investigated the effect of four ethanolic extracts of typified propolis collected in different Brazilian states, on Leishmania amazonensis performing assays with promastigote forms, extracellular amastigotes, and on infected peritoneal macrophages. Ethanolic extracts of all propolis samples (BRG, BRPG, BRP-1, and BRV) were capable to reduce parasite load as monitored by the percentage of infected macrophages and the number of intracellular parasites. BRV sample called red propolis, collected in the state of Alagoas, and containing high concentration of prenylated and benzophenones compounds, was the most active extract against L. amazonensis. The anti-Leishmania effect of BRV sample was increased in a concentration and time dependent manner. BRV treatment proved to be non-toxic to macrophage cultures. Since BRV extract at the concentration of 25 microg/ml reduced the parasite load of macrophages while presented no direct toxic to promastigotes and extracellular amastigotes, it was suggested that constituents of propolis intensify the mechanism of macrophage activation leading to killing of L. amazonensis. Our results demonstrate, for the first time, that ethanolic extracts of Brazilian propolis reduce L. amazonensis infection in macrophages, and encourage further studies of this natural compound in animal models of leishmaniasis.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/17426888
Author(s): Ramalingam K, Amaechi BT, Ralph RH, Lee VA
Author(s): Fukushima T, Iwao H, Nakazima A, Miki M, Sakai T, et al.
Author(s): Rokadiya S, Malden NJ
Author(s): Dessì A, Crisafulli M, Accossu S, Setzu V, Fanos V
Author(s): Santos FA, Bastos EM, Rodrigues PH, Uzeda M, Carvalho MAR, et al.
Author(s): Sreeja S, Babu PRS, Prathab AG
Author(s): Rams TE, Degener JE, van Winkelhoff AJ
Author(s): Cragg GM, Newman DJ, Snader KM
Author(s): Maekawa LE, Valera MC, Oliveira LD, Carvalho CA, Camargo CH, et al.
Author(s): Bonifácio BV, Silva PB, Ramos MA, Negri KM, Bauab TM, et al.
Author(s): Santos VR, Gomes RT, de Mesquita RA, de Moura MD, França EC, et al.
Author(s): Pereira EM, da Silva JL, Silva FF, De Luca MP, Ferreira EF, et al.
Author(s): Gomaa OM, Gaweesh AS
Author(s): Banskota AH, Tezuka Y, Kadota S
Author(s): Dornelas CA, Fechine-Jamacaru FV, Albuquerque IL, Magalhães HI, Dias TA, et al.
Author(s): Machado JL, Assunção AK, da Silva MC, Dos Reis AS, Costa GC, et al.
Author(s): de Oliveira PF, Leandro LF, Montanheiro G, Bastos JK, da Silva Filho AA, et al.
Author(s): Oliveira GA, Ferraz ER, Souza AO, Lourenço RA, Oliveira DP, et al.
Author(s): Righi AA, Alves TR, Negri G, Marques LM, Breyer H, et al.
Author(s): Teixeira EW, Negri G, Meira RM, Message D, Salatino A
Author(s): de Almeida EB, Cordeiro Cardoso J, Karla de Lima A, de Oliveira NL, de Pontes-Filho NT, et al.
Author(s): Sharaf S, Higazy A, Hebeish A
Author(s): Santos VR
Author(s): Park YK, Alencar SM, Aguiar CL
Author(s): Batista LL, Campesatto EA, Assis ML, Barbosa AP, Grillo LA, et al.
Author(s): Marcucci MC
Author(s): Gazzani G, Daglia M, Papetti A
Author(s): Santos VR, Pimenta FJ, Aguiar MC, do Carmo MA, Naves MD, et al.
Author(s): Samet N, Laurent C, Susarla SM, Samet-Rubinsteen N
Author(s): Jebahi S, Oudadesse H, Jardak N, Khayat I, Keskes H, et al.
Author(s): Rivadeneira J, Carina Audisio M, Boccaccini AR, Gorustovich AA
Author(s): Wei QW, Dong YM, Chen XF, Li YL, Miao GH
Author(s): Kaczmarek M, Jurczyk MU, Rubis B, Banaszak A, Kolecka A, et al.
Author(s): Andrade AL, Manzi D, Domingues RZ
Author(s): Alencar SM, Oldoni TL, Castro ML, Cabral IS, Costa-Neto CM, et al.
Author(s): Daugsch A, Moraes CS, Fort P, Park YK
Author(s): Trusheva B, Popova M, Bankova V, Simova S, Marcucci MC, et al.
Author(s): Miorin PL, Levy Junior NC, Custodio AR
Author(s): Park YK, Koo MH, Abreu JA, Ikegaki M, Cury JA, et al.
Author(s): Leitão DP, Filho AA, Polizello AC, Bastos JK, Spadaro AC
Author(s): Ferreira SBS, Assis Dias BR, Obregón CS, Gomes CC, de Araujo Pereira RR et al.
Author(s): Topcuoglu N, Ozan F, Ozyurt M, Kulekci G
Author(s): Ramos DBM, Gomes FS, Napoleão TH, Paiva PMG, Silva MDC, et al.
Author(s): Bueno-Silva B, Alencar SM, Koo H, Ikegaki M, Silva GV, et al.
Author(s): Libério SA, Pereira AL, Araújo MJ, Dutra RP, Nascimento FR, et al.
Author(s): Dziedzic A, Kubina R, Wojtyczka RD, KabaÅ‚a-Dzik A, Tanasiewicz M, et al.
Author(s): Jorgensen JH, Swenson JM, Tenover FC, Barry A, Ferraro MJ, et al.
Author(s): Gajan EB, Shirmohammadi A, Aghazadeh M, et al.
Author(s): Pondei K, Fente BG, Oladapo O
Author(s): Song XL, Sun YY, Liu Y, Chen Q, Bi XX, et al.
Author(s): Poptani B, Sharaff M, Archana G, Parekh V
Author(s): Cao WB, Su D, Chen YM, Zheng YZ, Zhang FK, et al.
Author(s): Olczak-Kowalczyk D, Daszkiewicz M, Krasuska-Slawinska, Dembowska-Baginska B, Gozdowski D, et al.
Author(s): Allan I, Newman H, Wilson M
Author(s): Krishnan V, Lakshmi T