Evaluation of antimicrobial, antioxidant and wound-healing potentials of Holoptelea integrifolia

Author(s): Srinivas Reddy B, Kiran Kumar Reddy R, Naidu VG, Madhusudhana K, Agwane SB, et al.


The methanolic extracts of Holoptelea integrifolia (Roxb.) (Urticaceae) leaves (MLE) and stem bark (MSBE) were studied for the wound-healing potential. Since wound healing is severely hampered by microbial infection and reactive oxygen species (ROS), this study was undertaken to evaluate antimicrobial and antioxidant activity apart from wound-healing activity. The antimicrobial property of the Holoptelea was studied against the six bacterial and five fungal strains using the agar well diffusion method and minimum microbicidal concentration and minimum inhibitory concentration were determined for each strain, in which methanolic extract of stem bark (MSBE) has shown bigger zone of inhibition (11.3-20.4 mm) than methanolic extract of leaves (MLE) (9.6-14.9 mm). The anti-oxidant activity was evaluated by DPPH free radical scavenging activity using HPLC method. The IC(50) values obtained for MSBE (TPC: 78.53+/-1.26 mg/g) and MLE (TPC: 57.71+/-1.45 mg/g) were 37.66+/-0.48 and 50.36+/-0.59 microg/well, respectively. In excision wound model, more than 90% wound healing was recorded in treated groups by 14 days of post surgery, where as only 62.99% was observed in the control group. In incision model, higher breaking strengths and higher hydroxyproline content in treated groups suggested higher collagen re-deposition than the control group. Finally, histopathology studies conformed wound-healing activity of Holoptelea integrifolia.

Similar Articles

Folklore medicinal plant of Mehwat (Gurgaon District), Haryana, India

Author(s): Sharma MP, Ahmad J, Hussain A, Khan S

Compendium of Indian Medicinal Plants (1970-1979)

Author(s): Rastogi RP, Melhothra BM

Studies on anti-inflammatory effect of aqueous extract of leaves of Holoptelea integrifolia, Planch

Author(s): Sharma S, Lakshmi KS, Patidar A, Chaudhary A, Dhaker S

Ethanolic leaf extract of Holoptelea integrifolia, Planch

Author(s): Shirinivas S, Ravindra K, Arandhana M, Kailash B

Ethnomedicine & Human welfare

Author(s): Khan IR, Khanum A

Screening of Nepalese medicinal plants for antiviral activity

Author(s): Rajbhandari M, Wegner U, Jülich M, Schöpke T, Mentel R