Extraction, Purification, and Inhibitory Effect of Alpha-Aylase Inhibitor from Wheat (Triticumaestivumvar

Author(s): Heidari R, Zareae S, Heidarizadeh M


Plant alpha-amylase inhibitors show great potential as tools to engineer resistance of crop plants against pests. They are also drug-design targets for treatment of diabetes and digestion disorderes. These inhibitors also known as sensitizing agents in human. The numerous form of alpha-amylase inhibitors was reported. In this study alpha-amylase inhibitor was extracted from Iranian wheat cultivar (Triticum aestivum v zarrin), precipitated and purified by anion exchange fast protein liquid chromatography. Electrophoresis of purified protein showed 0.66 relative mobility. Total hydrolytic activity of human salivary and bacillus subtilis alpha-amylase were inhibited 97.07% and 89.97% respectively by collected purified alpha-amylase inhibitor.

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