Author(s): Cheng Z, Moore J, Yu L
A high-throughput relative 2,2-diphenyl-1-picryhydrazyl (DPPH) radical scavenging capacity (RDSC) assay was developed and validated in the present study. This RDSC assay is easy to perform and has acceptable accuracy (90-110% recovery), precision [3.9-7.0% pooled relative standard deviation (RSD)], and reproducibility (2.2 and 3.5% interday and intraday RSD). This assay reports the RDSC values for antioxidant samples, which make it possible to compare the DPPH radical scavenging capacities of antioxidants determined in different laboratories. The RDSC assay may be conducted in aqueous alcohol and acetone for hydrophilic antioxidants or in the organic solvents for lipophilic antioxidants without solubilizing agents, which makes it possible to directly compare the radical scavenging capacities of hydrophilic and lipophilic antioxidants. In addition, the high-throughput RDSC assay could be utilized for EC50 value estimation. The high-throughput RDSC assay may be used for screening and investigating potential natural antioxidants.
Referred From: https://www.ncbi.nlm.nih.gov/pubmed/17002404
Author(s): Farnsworth NR
Author(s): Welna M, Klimpel M, Zyrnicki W
Author(s): Fraser GE, Sabaté J, Beeson WL, Strahan TM
Author(s): Yang J, Liu RH
Author(s): Amarowicz R, Troszynska A, Shahidi F
Author(s): Amaral JS, Casal S, Pereira JA, Seabra RM, Oliveira BP
Author(s): Khaled A, El Nemr A, El-Sikaily A, Abdelwahab O
Author(s): Malana MA, Ijaz S, Ashiq N
Author(s): Lopez-Ramon MV, Stoeckli F, Moreno-Castilla C, Carrasco-Marin F
Author(s): Lien EJ, Ren S, Bui HH, Wang R
Author(s): Yu L, Perret J, Harris M, Wilson J, Haley S
Author(s): Deshpande SS, Sathe SK, Salunkhe DK
Author(s): Sang S, Lapsley K, Jeong WS, Lachance PA, Ho CT, et al.
Author(s): Spiller GA, Jenkins DA, Bosello O, Gates JE, Cragen LN, et al.
Author(s): Koksal AI, Artik N, Simsek A, Gunes N
Author(s): Pinelo M, Rubilar M, Sineiro J
Author(s): Buldini PL, Ferri D, Sharma JL (1997 ) Determination of some inorganic species in edible vegetable oils and fats by ion chromatography
Author(s): Coco FL, Ceccon L, Ciraolo L, Novelli V
Author(s): Gulluce M, Sokmen M, Daferera D, Agar G, Ozkan H, et al.