HPLC Analysis of Flavonoids in Eupatorium littorale

Author(s): De Oliveira BH, Nakashimab T, Filhoc JS, Frehsea FL

Abstract

The isolation and identification of eupafolin and hispidulin in the dried leaves of Eupatorium littorale (Asteraceae) are described. A method for the detection of those flavonoids and rutin was also developed using reversed-phase HPLC with a photodiode array detector. Eupafolin and hispidulin were quantified and the concentrations found were 0.29% and 0.21%, respectively.

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