Insecticidal action of Annona coriacea lectin against the flour moth Anagasta kuehniella and the rice moth Corcyra cephalonica (Lepidoptera: Pyralidae)

Author(s): Coelho MB, Marangoni S, Macedo ML


Annona coriacea lectin (ACLEC) was tested for insecticidal activity against larvae of two pyralid moths, Anagasta kuehniella and Corcyra cephalonica. ACLEC produced approximately 50% mortality and mass loss in A. kuehniella larvae when incorporated into an artificial diet at levels of 1.5% and 1.0% (w/w), respectively. In contrast, the inclusion of up to 2% ACLEC in the diet did not significantly decrease the survival or weight of C. cephalonica larvae. The nutritional indices for A. kuehniella and C. cephalonica suggested that ACLEC had a multi-mechanistic mode of action and was an antifeedant for both insects. The toxicity in A. kuehniella apparently resulted from a change in the gut membrane environment and consequent disruption of digestive enzyme recycling mechanisms. Affinity chromatography showed that ACLEC bound to midgut proteins of A. kuehniella and C. cephalonica. However, the 14 kDa subunit of ACLEC was not digested by midgut proteases of A. kuehniella, but was degraded by the corresponding C. cephalonica proteases within a few hours. These findings suggest the possibility of using ACLEC to engineer crop plants.

Similar Articles

Molluscidal properties of some species of Annona

Author(s): Dos Santos AF, Sant’ana AE

Pure annonin and a process for the preparation thereof, US Patent 4689232

Author(s): Moeschler HF, Pfluger W, Wendisch D

Control of pests with annonaceous acetogenins, US Patent 4721727

Author(s): Mikolajczak KJ, McLaughlin JL, Rupprecht JK

Control of pests with annonaceous acetogenins, US Patent 4721727

Author(s): Mikolajczak KJ, McLaughlin JL, Rupprecht JK

Acetogenins from Annona glabra seeds, Phytochemistry 47: 811-816

Author(s): Teresa G, Raul A, Jose RT, Amparo MB, Carmen ZMP

Comparative efficacy of Annona squamosa Linn

Author(s): George S, Vincent S

Selective action of acetogenin mitochondrial complex I inhibitors

Author(s): González-Coloma A, Guadaño A, de Inés C, Martínez-Díaz R, Cortes D

Insecticidal and mutagenic evaluation of two annonaceous acetogenins

Author(s): Guadaño A, Gutiérrez C, de La Peña E, Cortes D, González-Coloma A

Medical Insects and Arachnids

Author(s): Crosskey RW, Lane RP

Protein measurement with the Folin phenol reagent

Author(s): Lowry OH, Rosebrough NJ, Farr AL, Randall RJ

Acetogenins from Annonaceae, inhibitors of mitochondrial complex I

Author(s): Zafra-Polo MC, González MC, Estornell E, Sahpaz S, Cortes D

Flics and disease

Author(s): Greenberg B