Investigation of the in vivo organization of anthocyanins using resonance raman microspectrometry

Author(s): Merlin JC, Statoua A, Brouillard R


Resonance Raman (RR) microspectrometry constitutes a new means for studying the organization of anthocyanins in living tissues. RR spectra of the pigments present in a single cell have been recorded from the skins of the mature berries of the ‘Pinot noir’ grape, as well as the petals of the common mallow. Comparison of these spectra with those obtained from model solutions of anthocyanins permits us to conclude that in the ‘Pinot noir’ berries, malvidin 3-glucoside is the main pigment. Furthermore, inside the skin it is essentially in the quinonoidal base form, whereas in the outer face of the skin it is mainly in the flavylium form. In the upper epidermis of petals of the common mallow, only malvidin 3,5-diglucoside could be detected, entirely in the cationic flavylium form. Since self-association or co-pigmentation processes do not seem to contribute much to the RR spectra, we conclude that they perturb the electronic excited state of the monomeric anthocyanin chromophores much more than they modify the corresponding electronic ground state. In the case of the skins of the mature berries of the ‘Pinot noir’ grape, dark grains, which we believe to be anthocyanoplasts, have been observed. In vivo RR spectra of the dark grains have been recorded.

Similar Articles

Nutritional assessment of yam (Dioscorea alata) tubers

Author(s): Wanasundera JP, Ravindran G

Estrogen in yams

Author(s): Mirkin G

Effects of Taiwanese yam (Dioscorea japonica Thunb var

Author(s): Chen HL, Wang CH, Chang CT, Wang TC

Antioxidative activity of dioscorea and dehydroepiandrosterone (DHEA) in older humans

Author(s): Araghiniknam M, Chung S, Nelson-White T, Eskeison C, Watson RR

Both dioscorin, the tuber storage protein of yam (Dioscorea alata cv

Author(s): Hsu FL, Lin YH, Lee MH, Lin CL, Hou WC

Resonance raman spectroscopic studies of anthocyanins and anthocyanidins in aqueous solutions

Author(s): Merlin JC, Statous A, Cornard JP, Saidi-Idrissi M, Brouillard R

Non-destructive NIR FT Raman analysis of plants

Author(s): Schrader B, Klump HH, Schenzel K, Schulz H

In Situ and In Vitro Antioxidant Activity of Sweetpotato Anthocyanins

Author(s): Philpott M, Gould KS, Lim C, Ferguson LR

Two acylated anthocyanins from purple sweet potato

Author(s): Goda Y, Shimizu T, Kato Y, Nakamura M, Maitani T, et al.

Enzymes of Ureide Synthesis in Pea and Soybean

Author(s): Christensen TM, Jochimsen BU

Egg science and technology

Author(s): Stadelman WJ, Cotterill OJ