Involvement of nitric oxide in the gastroprotective effects of an aqueous extract of Pfaffia glomerata (Spreng) Pedersen, Amaranthaceae, in rats

Author(s): Freitas CS, Baggio CH, Da Silva-Santos JE, Rieck L, de Moraes Santos CA, et al.

Abstract

The plants belonging to Pfaffia genus are used in folk medicine to treat gastric disturbances. This study examined the effects of an aqueous extract of Pfaffia glomerata (Spreng) Pedersen (AEP) on the gastrointestinal tract. Wistar rats were pretreated orally (p.o.) with the AEP (125, 250, 500 and 1000 mg.kg(-1)) before induction of ulcers by hypothermic restraint stress (HRS, 3 h restraint stress at 4 degrees C), ethanol (ET, 70%; 0.5 ml/animal; p.o.) or indomethacin (IND, 20 mg.kg(-1); s.c.). Control animals received water (C) or ranitidine (60 mg.kg(-1)) p.o. The AEP protected rats against HRS and ET-induced ulcers, but was not able to protect the gastric mucosa against IND-induced ulcers. When injected into the duodenal lumen, the AEP reduced total acidity and both basal and histamine-stimulated acid secretion in pylorus-ligated rats. In addition, gastric secretion from AEP-treated animals exhibited increased concentrations of nitrite and nitrate. Treatment of animals with L-NAME (120 mg.kg(-1), p.o.) prevented both the reduction of total acidity and the increase in NOx levels promoted by AEP treatment. In conclusion, AEP effectively protected the gastric mucosa and inhibited gastric acid secretion in rats, probably by involving the histaminergic pathway and an enhanced production of nitric oxide in the stomach.

Similar Articles

Ecdysteroids and a sucrose phenylpropanoid ester from Froelichia floridana

Author(s): Wang P, Li S, Ownby S, Zhang Z, Yuan W, et al.

Isolation and HPLC quantitative analysis of antioxidant flavonoids from Alternanthera tenella Colla

Author(s): Salvador MJ, Ferreira EO, Mertens-Talcott SU, De Castro WV, Butterweck V, et al.

Triterpene saponins from Alternanthera repens

Author(s): Sanoko R, Esperanza G, Pizza C, De Tommasi N

Phytochemical Methods

Author(s): Harbone JB

Iridoid glucosides with free radical scavenging properties from fagraea blumei

Author(s): Cuendet M, Hostettman K, Potterat O, Dyatmko W

The chemistry behind antioxidant capacity assays

Author(s): Huang D, Ou B, Prior RL

Antioxidant capacity and phenolic content of four Myrtaceae plants of the south of Brazil

Author(s): Salvador MJ, de Lourenço CC, Andreazza NL, Pascoal AC, Stefanello ME

New colorimetric cytotoxicity assay for anticancer-drug screening

Author(s): Skehan P, Storeng R, Scudiero D, Monks A, McMahon J, et al.

Flavonoids as antioxidants

Author(s): Pietta PG

Antioxidant capacity of extracts and isolated compounds from Stryphnodendron obovatum Benth

Author(s): Zocoler AMD, Sanches ACC, Albrecht I, Mello JCP

Antioxidant and cytotoxic activities of carob tree fruit pulps are strongly influenced by gender and cultivar

Author(s): Custodio L, Fernandes E, Escapa AL, Fajardo A, Aligue R, et al.

Pfaffia paniculata (Brazilian ginseng) methanolic extract reduces angiogenesis in mice

Author(s): Carneiro CS, Costa-Pinto FA, da Silva AP, Pinello KC, da Silva TC, et al.

Vitexin, an HIF-1alpha inhibitor, has anti-metastatic potential in PC12 cells

Author(s): Choi HJ, Eun JS, Kim BG, Kim SY, Jeon H, et al.

Vitexins, nature-derived lignan compounds, induce apoptosis and suppress tumor growth

Author(s): Zhou Y, Liu YE, Cao J, Zeng G, Shen C, et al.