Author(s): Moustafa YAM, Khodair AI, Saleh MA
An investigation of Leptadenia pyrotechnica (Forsk.) Decne (Asclepiadaceace) chemical constituents led to the isolation of six flavonoids, kaempferol-3-O-α-l-rhamnopyranosyl (1′″→6″)-O-β-d-glucopyranoside (E-I.1), kaempferol-3-O-β-d-rhamnopyranosyl (1′″→6″)-O-β-d-glucopyranoside (E-I.2), texasin-7-O-β-d-glucopyranoside E-II.2, kaempferol-3-O-β-d-glucopyranoside (E-III.1), kaempferol (E-IV.1) and kaempferide-3-O-α-l-rhamnopyranosyl (1′″→6″)-O-β-d-glucopyranoside (E-I.1a). The isolation of these compounds was carried out using Sephadex LH-20 low pressure liquid chromatography (LPLC), preparative paper chromatography (PC), and high performance liquid chromatography (HPLC). The chemical structures of the isolated compounds were established by mass spectrometry (FAB- and EI- techniques), nuclear magnetic resonance NMR (1H-, 13C- and COSY) spectral data and ultraviolet (UV) spectroscopic techniques. The acute toxicity of total alcoholic and total flavonoid extracts were examined by brine shrimp. The LC50 values were 11.89 and 84.14 ppm for the total alcoholic and total flavonoid extracts, respectively. The mortality rates of the isolated flavonoid fractions of E-I, E-I.1, E-I.2 represent the higher percentages of mortality compared with the rest of the flavonoid fractions. The plant exhibited activity as an antitumor agent in the initial potato disc screen.
Author(s): Jenkinson F, Steele RJ
Author(s): Watson AJ
Author(s): Watson AJ1
Author(s): Pan L, Chai H, Kinghorn AD
Author(s): El Ghonemy AA
Author(s): Jongbloed M
Author(s): Hammiche V, Maiza K
Author(s): Cioffi G, Sanogo R, Vassallo A, Dal Piaz F, Autore G, et al.
Author(s): Nishant V, Jha KK, Sudhir C, Omvir S, Arvind K
Author(s): Alqasoumi SI, Soliman GAE, Awaad AS, Donia AEM
Author(s): Khasawneh MA, Elwy HM, Hamza AA, Fawzi NM, Hassan AH
Author(s): Khan N, Adhami VM, Mukhtar H
Author(s): Erel O
Author(s): Yu T, MacPhail SH, BanĂ¡th JP, Klokov D, Olive PL
Author(s): Shu KX, Li B, Wu LX
Author(s): Donovan M, Cotter TG
Author(s): Turk B, Stoka V
Author(s): Coultas L, Strasser A
Author(s): Oliver L, Vallette FM
Author(s): Lambert JD, Elias RJ
Author(s): Li GX, Chen YK, Hou Z, Xiao H, Jin H, et al.
Author(s): Saleem M
Author(s): Liu F, He Y, Liang Y, Wen L, Zhu Y, et al.
Author(s): Siddique HR, Saleem M
Author(s): Fatma EG
Author(s): Reddy LH, Couvreur P
Author(s): Block S, Baccelli C, Tinant B, Van Meervelt L, Rozenberg R, et al.
Author(s): Bhattacharya A, Banu J, Rahman M, Causey J, Fernandes G