Author(s): Drira R, Chen S, Sakamoto K
Aims: Oleuropein and hydroxytyrosol, which are antioxidant molecules found in olive leaves and oil, have been reported to exert several biochemical and pharmacological effects. These polyphenols are able to prevent low-density lipoprotein oxidation and protect cells against several diseases. Here, we studied the effect of these compounds on adipocyte differentiation in 3 T3-L1.
Main methods: To perform this study, 3 T3-L1 preadipocytes viability was analysed via Trypan blue and MTT assays, and triglycerides were stained with Oil Red O. Adipogenesis related genes expression were checked by RT-PCR and qRT-PCR. Also, cells counting and flow cytometry were used to analyse the mitotic cell cycle during the adipogenesis clonal expansion phase.
Referred From: https://pubmed.ncbi.nlm.nih.gov/21945192/
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